Figure 3

Increased frequency of cell death induced in root tips of osrecql4 mutants upon aphidicolin treatment. Propidium iodide (PI) staining indicates dead cells. (A) WT and osrecql4-2 mutant plants treated with 40 mg/L aphidicolin for 24 h. An increased number of cell death signals was observed in the root meristem of osrecql4-2 under the fluorescence microscope. Scale bar, 100 μm. (B) Summary of number of PI signals. 24 independent roots from osrecql4-2 and WT plants were investigated. The x-axis indicates number of cell death spots per root. SD, standard deviation.