Figure 5

DNA breaks are induced in the root meristem of osrecql4-2 mutant plants upon aphidicolin treatment. The TUNEL assay detects single- and double-strand DNA breaks and nicks via 3'-OH end labeling. Following 40 mg/L aphidicolin treatment for 24 h, the number of DNA damage signals seen in root meristems of osrecql4-2 under the fluorescent microscope was observed. DNA damage signals in the epidermal cells seem to be induced by light or physical stress. Scale bar, 100 μm.