Figure 6

Enhanced HR in osrecql4-2 mutants. (A) The GUS recombination reporter (pGU.C.US) used in this experiment is illustrated schematically. Recombination between the two overlapping sequences produces a functional GUS gene. (B) Graph showing the number of GUS blue spots per callus weight as counted under the microscope. HR frequency increased more in the osrecql4-2 mutant than in the WT. GUS histochemical staining was performed on 17-day-old calli. (C)Aphidicolin treatment enhanced HR further in osrecql4-2 mutants, but HR frequency in WT did not differ from that of the WT without aphidicolin treatment. 17-day-old calli were treated 5 mg/L aphidicolin for 24 h. The aphidicolin-treated calli were then subjected to GUS staining. Blue diamonds OsRecQl4(+/+); red squares OsRecQl4(−/−); blue solid line average of OsRecQl4(+/+) GUS spots; red solid line average of OsRecQl4(−/−) GUS spots; blue triangles aphidicolin treated OsRecQl4(+/+), red circles aphidicolin treated OsRecQl4(−/−); blue dotted lines average of aphidicolin-treated OsRecQl4(+/+) GUS spots; red dotted line average of aphidicolin-treated OsRecQl4(−/−) GUS spots.