Figure 10

Broadly targeted metabolomic analysis of herbivore-induced EV and MYC2-VIGS plants. Leaves (n=3) were collected from caterpillar-attacked (4 d) EV and MYC2-VIGS plants and used for untargeted metabolomic analysis with an HPLC-TOF-MS. Raw data were pre-processed by XCMS and CAMERA packages and a PCA plot (A) was generated based on the molecular features that differed significantly (fold change > 2, P < 0.05) among the indicated genotypes. Principal component 1 (PC 1) explains 51.7% of the variance while PC 2 explains 27.5%. The contribution of the molecular features to the PCA clusters is shown by the loading plot (B) while the volcano plot (D) depicts important features with a fold change and t-test threshold of 2 and 0.1, respectively. (C) A heat map depicts the expression of regulated molecular features in EV and MYC2-silenced plants.