Functional genomics of a generalist parasitic plant: Laser microdissection of host-parasite interface reveals host-specific patterns of parasite gene expression

Table 1 Read and assembly level statistics for T. versicolor interface transcriptomes

Low quality reads were filtered before assembly, and host sequences were filtered both before and after assembly. Unigenes remaining after removal of host plant and non-plant sequences were aligned with BLASTx to sequences detected in any other PPGP transcriptome library of Triphysaria versicolor (http://ppgp.huck.psu.edu/). Unigenes with less than 95% pairwise identity to either host or to other Triphysaria libraries were sorted further if a BLASTx search of the NR (http://www.ncbi.nlm.nih.gov) database yielded alignments of 1e-10 or stronger. The remaining unclassified unigenes were submitted to OrthoMCL DB and InterProScan. Unigenes that remained unclassified after the final screen are called “no hit” unigenes.

ISSN: 1471-2229