Genetic analysis of tolerance to the root lesion nematode Pratylenchus neglectus in the legume Medicago littoralis

Table 1 SSR and CAPS markers closely linked to QPnTolMl.1

Marker name as in Figure4	Primer sequences (5′ – 3′)	Amplicon size (genomic)	LOD^a	% Var^b
AC122725*	F: CCTGGCACGAGTCATGTAAC	ca. 250 bp	15.8	41
AC122725*	R: TCACGAGTTTTCAAATTTATCAT	ca. 250 bp	15.8	41
STkinBsrI	F: GTGAAAAGCATGCTGCGGAA	913 bp	19.9	49
STkinBsrI	R: AAAGGCAAAGGACCACACCA	913 bp	19.9	49
SatNlaIII	F: TGCCAACCTCCCTCCAATTC	1072 bp	19.4	48
SatNlaIII	R: AAAGGCAAAGGACCACACCA	1072 bp	19.4	48
LTP2TaqI	F: ACGATCACTAGCTTGAGCCC	933 bp	18.7	47
LTP2TaqI	R: TCTTACTCTGGAAAGCGATTACA	933 bp	18.7	47
XylnHincII	F: CAGGCCAAAAGCCCTTGTTC	948 bp	18.4	46
XylnHincII	R: CCCAATCGTGTCCCAGTCAA	948 bp	18.4	46
AC74HinfI	F: GGAGAAACTTGCAGAGCAGGCC	1264 bp	14.1	37
	R: ACGATCCATCAGGAACTCCCGT

*Accession ID: MtYoungUMinn2006_1_h2_4c11e at cmap.comparative-legumes.org; ^aLogarithm of odds, ^bpercentage of genetic variation explained by locus.

ISSN: 1471-2229