Figure 8

Quantitative RT-PCR analysis of flowering genes downstream of JcFT in WT and 35S:: JcFT transgenic Jatropha . The qRT-PCR results were obtained using two independent biological replicates and three technical replicates for each RNA sample extracted from apex of the 35S::JcFT transgenic and wild-type (WT) shoots cultured in vitro. Transcript levels were normalized using JcActin1 gene as a reference. The mRNA level in WT was set as the standard with a value of 1.