Figure 5

Ethylene insensitive-related phenotypes of 2HA plants. Bending of cotyledons on P40 media supplemented with ACC. 2HA plants (a) and Jemalong plants (b) germinated on P40 media with 0.5 μM ACC. Graph (c) represents % of seedlings with bent cotyledons. Cotyledons that formed an angle of < 90° in relation to the stalk are counted as bent. Results are mean ± SE of 3 biological repeats. Bars for sickle plants are not shown because they are not bent at any concentration of ACC. (d) Root growth on P4 medium. Seven days old seedlings of sickle mutant, 2HA line and WT Jemalong are presented in duplicate. Bar is 1 cm. (e) Root length (mean ± SE in mm) of seven day old seedlings grown on P4 medium (n = 90).