Table 3 Segregation of flower color in F 1 plants and F 2 populations derived from crosses between soybean cultivar Clark or near-isogenic lines (Harosoy- w4 and Clark- w4 ) and flower color variants, Glycine soja accession, kw4, and soybean mutant lines, 222-A-3 and E30-D-1 in Tsukuba, Japan
From: Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2
Generation | Year | Number of plants | Expected ratio | χ2value | Probability (Pvalue) | |||
|---|---|---|---|---|---|---|---|---|
Total | Purple | Light purple | Near white | |||||
kw4 | 2009 | 10 | - | - | 10 | - | - | - |
Harosoy-w4 (H-w4) | 2009 | 10 | - | - | 10 | - | - | - |
H-w4 × kw4 F1 | 2009 | 5 | - | - | 5 | - | - | - |
H-w4 × kw4 F2 | 2009 | 116 | - | - | 116 | - | - | - |
222-A-3 (222) | 2010 | 10 | - | - | 10 | - | - | - |
Clark-w4 (C-w4) | 2010 | 10 | - | - | 10 | - | - | - |
222 × C-w4 F1 | 2010 | 5 | - | - | 5 | - | - | - |
222 × C-w4 F2 | 2010 | 109 | - | - | 109 | - | - | - |
E30-D-1 (E) | 2010 | 10 | - | 10 | - | - | - | - |
Clark (C) | 2010 | 10 | 10 | - | - | - | - | - |
E × C F1 | 2010 | 4 | 4 | - | - | - | - | - |
E × C F2 | 2013 | 112 | 84 | 28 | - | 3:1 | 0.00 | 1.00 |
E × C-w4 F1 | 2010 | 3 | - | 3 | - | - | - | - |
E × C-w4 F2 | 2010 | 111 | - | 82 | 29 | 3:1 | 0.08 | 0.78 |