Table 2 CODEHOP primers used for UGTs amplification. y = [C, T], n = [A, G, C, T], r = [A, G], w = [A, T], k = [G, T]. BLAST analysis of protein consensus sequences corresponding to each primer was performed as indicated in the legend of Table 1.
From: Conservation and diversity of gene families explored using the CODEHOP strategy in higher plants
Primer | Sequence | Protein | BLASTp hits on arabidopsis UGT database |
|---|---|---|---|
Sense | Â | Â | Â |
   PUGTa | TG.TGC.GAG.TCC.ACC.ATG.aar.may.tgy.yt | LCESTMKNCL | 85A |
   PUGTb | GCC.GAG.GAG.CTC.GGC.rtn.ccn.gar.gt | AEELGVPEV | 85A |
   PUGTc | CC.TTC.TGC.CGG.ACC.ACC.ray.ccn.gay.ga | SFCRTTNPDD | 85A |
   PUGTd | CT.ATG.CAG.TCC.ATC.TTC.ccn.ccn.rtn.ta | AMQSIFPPVY | 85A |
   PUGTe | G.CCG.GAC.GTG.GTG.GCn.ggn.gan.gar.g | LCESTMKNCL | 85A |
Reverse | Â | Â | Â |
   PUGTar | GC.GGA.GGT.GGT.CCA.GAA.Cwr.nac.ytc.ngg | PEVQFWTTS | 85A |
   PUGTbr | GAA.GTT.GAC.CAT.GAC.GTC.Gtc.ngg.rty.ngt | TNPDDVMVSF | 85A |
   PUGTcr | CGG.GCC.GAC.GGT.Gta.nay.ngg.ngg | PPVYSVGP | 85A/71B/71C/72B |
   PUGTdr | CGG.CAC.CAT.GGC.Ctc.ntc.ncc.nkc | AGEEAMVP | 85A |
   PUGTer | CCA.CTC.GTC.GCA.GCA.GAA.Cyk.rca.rtt.nrt | MNCKFCCDEW | 85A |