Rapid and accurate pyrosequencing of angiosperm plastid genomes

BMC Plant Biology

Table 4 Error rates for the GS 20 plastid genome sequence

Region	Nandina	Platanus	combined
overall genome	0.043	0.031	0.037
overall SC	0.030	0.064	0.047
overall IR	0.054	0.004	0.029
overall coding	0.027	0.029	0.028
overall noncoding	0.085	0.036	0.062
SC coding	0.036	0.055	0.046
SC noncoding	0.000	0.161	0.057
IR coding	0.018	0.000	0.009
IR noncoding	0.115	0.011	0.063

Observed error rates for the GS 20 plastid genome sequence of Nandina, Platanus, and both genomes combined (given in percent). These error rates are based on known GS 20 errors discovered in regions of conventional comparison sequence. Only one copy of the IR was included in error calculation.

ISSN: 1471-2229