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Figure 3 | BMC Plant Biology

Figure 3

From: Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L

Figure 3

Expression of recombinant HCT in E. coli. Protein content of non-induced (T = 0 h) and induced (T = 8 h) non-transformed cells (1) were compared with those of non-induced (T = 0 h) and induced (T = 8 h) transformed cells (2). After induction at 37°C for 8 h, HCT protein cannot be detected in the soluble fraction (2 S at T = 8 h), but is present in the soluble fraction after induction at 28°C. Arrows indicate the ~50 kDa HCT protein. Molecular marker masses indicated on the left. (1 = empty vector; 2 = vector -HCT; S = soluble fraction; P = pellet; M = molecular weight marker).

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BMC Plant Biology

ISSN: 1471-2229

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