Figure 1

Expression of rolAp-iaaMtms2 construct in indeterminate root nodules. A. Schematic drawing of the chimeric operon. Restriction endonuclease sites used for chimeric operon construction are reported. B. Agarose gel electrophoresis of RT-PCR product obtained from total RNA extracted from nodules (lanes 1 and 5) formed by S. meliloti IAA strain in M. truncatula and M. sativa, respectively. Lanes 3 and 7, RT-PCR performed on total RNA extracted from nodules induced by the control strain in M. truncatula and M. sativa, respectively. Lanes 2 and 4, RNA from nodules of M. truncatula induced by IAA and control strain, amplified without reverse transcriptase; lanes 6 and 8, RNA from nodules of M. sativa induced by IAA and control strain, amplified without reverse transcriptase. Lane 9, no-template control. The position of the primers used in RT-PCR analysis is indicated by arrows in the schematic drawing reported in panel A.