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Figure 3 | BMC Plant Biology

Figure 3

From: A strong constitutive ethylene-response phenotype conferred on Arabidopsis plants containing null mutations in the ethylene receptors ETR1 and ERS1

Figure 3

The etr1-7;ers1-3 and etr1-9;ers1-3 mutants exhibit a severe constitutive ethylene-response phenotype in dark-grown seedlings. (A)Effect of single and double mutations of subfamily 1 receptors upon seedling growth. etr1-7 was isolated from ecotype Columbia (Col), whereas etr1-9, ers1-2, and ers1-3 were isolated from ecotype Wassilewskija (WS). Both Col and WS wild-type seedlings are included as controls. Seedlings were grown in the dark for 3.5 days in the absence (air) or in the presence of ethylene (10 μL/L). AVG (5 μM) was included in growth media to inhibit ethylene biosynthesis by the seedlings. Mean hypocotyl lengths are given in millimeters with SD in parentheses. (B) Rescue of the constitutive ethylene-response phenotype of etr1-9;ers1-3 by transformation with wild-type ETR1 (tETR1). Phenotypes of 4-day-old seedlings are shown for a wild-type control, the etr1-9;ers1-3 mutant line, and two independent lines of etr1-9;ers1-3 transformed with tETR1.

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