Figure 4

Schematic of relevant chimeric and non-chimeric orfs generated by the wp allele. In order of decreasing aa length the chimeric orfs from several of the chimeric mRNAs isolated were: A (418 aa) containing F3H Exons 1 and 2, the UP and CDC2 Exons; C (293 aa) with F3H Exon 1, 3'end 16 aa, and Exon 2 plus UP and CDC2 Exons; D (143 aa) had 9 aa of the UP intron plus the UP and CDC2 Exons. E (105 aa) with 34 aa of the FPK/MDH Intron plus 71 aa of the FPK Exon; F (105 aa) had 19 aa of FPK Intron, 77 aa of FPK Exon and 9 aa of MDH Exon. The non chimeric orf B (394 aa) had the three F3H Exons identical to the ones translated from the Wp allele the only cDNA clone with this orf was wp-15s. The chimeric orfs were generated from several of the cDNAs sequenced and they are listed underneath each orf class and also the frame in each one of the clones. * The orf from the wp-6c clone was 5 aa shorter. ** The orf from the wp-6c was 2 aa longer.