Figure 6

Short myosin XI tail length fusion constructs are insufficient to be targeted to organelles. Tobacco leaf cells after Agrobacterium infiltration with different myosin tail length fusion constructs. A-D) Complete tail constructs. E-G) 1/2 coil constructs. H-J) Nocoil constructs. K-N) 1/2 tail constructs. O-R) Dilute constructs. The yellow signal is from the YFP fusion constructs, red is chlorophyll autofluorescence. The shorter the tail, the more unspecific cytoplasmic labeling is observed. Rather few punctuate structures were observed in cells with 1/2 tail or dilute constructs. n/d = not determined. Bar = 10 μm.