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Table 2 Brachypodium reference genes for normalization and their expression stability values calculated by the NormFinder software

From: Exploring valid reference genes for gene expression studies in Brachypodium distachyonby real-time PCR

Rank Tissue Growth hormone Heat/Cold High salt/Drought Developmental stage Total
  Gene Stability Gene Stability Gene Stability Gene Stability Gene Stability Gene Stability
1 Ubi4 0.0846 Ubi10 0.1377 GAPDH 0.1723 SamDC 0.0372 Ubi4 0.0987 UBC18 0.2586
2 Ubi10 0.0846 Ubi4 0.1640 EF1α 0.1951 UBC18 0.0378 SamDC 0.0996 Ubi4 0.3363
3 GAPDH 0.2751 GAPDH 0.1768 SamDC 0.2164 GAPDH 0.0738 UBC18 0.156 EF1α 0.3876
4 EF1α 0.2796 ACT7 0.2053 ACT7 0.2434 EF1α 0.1951 GAPDH 0.2276 SamDC 0.4791
5 ACT7 0.3708 TUA6 0.2103 RCA 0.2689 RCA 0.2063 ACT7 0.3401 ACT7 0.4845
6 UBC18 0.5032 UBC18 0.2291 Ubi10 0.3139 Ubi10 0.3426 RCA 0.3682 TUA6 0.5777
7 SamDC 0.5512 EF1α 0.3028 TUA6 0.3279 ACT7 0.3441 Ubi10 0.3776 Ubi10 0.5937
8 RCA 0.8238 SamDC 0.3946 UBC18 0.3309 Ubi4 0.4078 EF1α 0.6029 GAPDH 0.6658
9 TUA6 1.3712 RCA 0.7124 Ubi4 0.8483 TUA6 0.7401 TUA6 0.6579 RCA 0.926
  1. In each column, a low stability value as an estimate of the combined intra- and intergroup variation of the respective gene represents a high gene expression stability of the respective gene between the matched samples.