Table 2 Monitored stress: experimental system on propagation plugs
From: ESKIMO1 is a key gene involved in water economy as well as cold acclimation and salt tolerance
At time 0, propagation plug saturation was 100% (SMWC = Substrate maximum water content) | |||
|---|---|---|---|
Propagation plug saturation | 60% Control | 30% Mild water deficit | 20% Severe water deficit |
Averaged determination | All the plants were watered daily to reach 60% SMWC with a volume based on the average weight of a subset of propagation plugs | All the plants were watered daily to reach 30% SMWC with a volume based on the average weight of a subset of propagation plugs | Not done (threshold effect) |
Individual determination | Each plant was watered daily to reach 60% SMWC based on the actual weight of the propagation plug | Each plant was watered daily to reach 30% SMWC based on the actual weight of the propagation plug | Each plant was watered daily to reach 20% SMWC based on the actual weight of the propagation plug |
Salt stress: individual determination | Each plant was watered daily to reach 60% SMWC based on the actual weight of the propagation plug. Once at 60%, salt stress was applied with 0.5× a nutritive solution supplemented with 150 mM NaCl. | Not done (combined water deficit and salt stress not applied) | Not done (combined water deficit and salt stress not applied) |