Figure 5

RT-PCR to detect transcripts of thirteen novel mycorrhiza-induced M. truncatula genes in colonized cortical cells. RNA from cortical cells from three independent mock-inoculated M. truncatula root systems (LM-M1, LM-M2 and LM-M3) and cortical cells containing arbuscules from M. truncatula/G. versiforme mycorrhizal root systems (LM-Gv1 and LM-Gv2) or M. truncatula/G. intraradices (LM-Gi3) mycorrhizal root systems was used. Cortical cells were obtained by laser microdissection (LM). Transcripts that were induced in only one of the M. truncatula/G. versiforme LM samples were further evaluated in M. truncatula/G. intraradices LM samples. The larger size fragment in LM-Gi3 (TC97569) may indicate presence of another peptide transporter gene. TC106485 was used as endogenous control.