Figure 2
Immuno-gold labeling and electron microscopic observation showed that AtKinesin-13A was located on Golgi-associated vesicle in root cap cells of Arabidopsis. AtKinesin-13A was labeled with the purified AtKinesin-13A antibody. The AtKinesin-13A antibody was detected with a secondary antibody with 10 nm gold particles. (A) Electron microscopic observation showed that AtKinesin-13A was located mainly on Golgi-associated vesicle in root cap cells of Arabidopsis. (B) Note the labeling on the margin of Golgi vesicles in Arabidopsis root cap cells (arrows). (C) Control section, incubated with the secondary antibody alone, did not show gold particles association with Golgi vesicles. (D) Atkinesin-13A antibody could not label Golgi vesicles in the section of root cap cells in kinesin-13a-1 mutant line. G: Golgi apparatus. SV: secretory vesicles. Bars: 200 nm (A, D); 150 nm (B, C).