Figure 5

Effects of hormone treatments in P-miR164a activity in transgenic Arabidopsis plants. (A) L56, and L35 P-miR164a::GUS Arabidopsis transgenic lines and 35S::GUS control transgenic plants were hormone-treated as indicated in methods section. The bar chart shows the GUS activity mean value and the standard errors of results obtained in each group with n ≥ 10 from at least two biological replicates. Values were normalized to mock-treated controls of each line. Statistical comparisons were made by the Kruskal-Wallis test with Dunn's post-test. Statistical differences between treated and mock-treated groups are shown. **p < 0.05. (B) Effectiveness of hormone treatments by amplifying mRNAs that are known to be hormone inducible as ABA inducible RD22 (NM_122472); IAA inducible SAUR-AC1 (S70188) and GA3 inducible APT1 (NM_179383) genes. ACTIN2 gene was also amplified as internal control. M: 1 Kb DNA molecular marker; (-) Negative PCR control (without DNA).