Figure 5

Molecular analysis of selected primary clones. (a) analysis of T-DNA insertions by Southern hybridization of total genomic DNA digested with either BamHI (B) or HindIII (H). (b) Methylation analysis by Southern hybridization of total genomic DNA digested with HindIII (H) and EcoRI (E), cleaving out the 35S-GFP cassette, and further with methylation-sensitive endocucleases Bsu15I, Eco72I or Eco47I, having restriction sites within the cassette; arrows indicate position of uncleaved 35S-GFP cassette. The blots were hybridised with DIG-dUTP-labelled GFP probe. M, molecular weight ladder.