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Table 3 Marker validation and features of new 61 UGMS markers of tea

From: Identification, characterization and utilization of unigene derived microsatellite markers in tea (Camellia sinensis L.)

     

Heterozygosity **

   

Locus

name *

Primer sequence

Repeat Motif

Annealing temperature

(T a )

No. of alleles

H O

H E

PIC

Approximate size range (bp)

No. of genotypes amplified

TUGMS1

F5’CTTCAAGTTGAGTTTGTCCG'

R5'CAAGGGATGGTTTTCACTTG

(TA)13

55°C

4

0.118

0.558

0.770

85 bp–100 bp

15

TUGMS3

F5'GCGTATGGAAAAGCTGAGAA3'

R5'GAAGCAAACCACTGAGGTGA3'

(TC)10

57°C

8

0.559

0.857

0.595

160 bp–220 bp

34

TUGMS4

F5'CCACCGACTCGATGACATAA3'

R5'GCATTGAGATTGATGGACCA3'

(TC)11(CA)11

57°C

6

0.765

0.808

0.306

250 bp–300 bp

32

TUGMS7

F5'GGACCACTTGATTTTCAGCT3'

R5'ACGTACAATCACCACCGACT3'

(GA)19

55°C

6

0.853

0.766

0.154

300 bp–400 bp

34

TUGMS11

F5'GGGGAGTGTTTGTTTGAATA3'

R5'TGTAGGGTTCTTTGAGGCAG3'

(GA)22

55°C

8

0.500

0.857

0.630

190 bp–240 bp

29

TUGMS12

F5'GAAGTTTGTTGAGAGTGCTGC3'

R5'ACAGATCTAAATTTGGGGGG3'

(TA)22

55°C

7

0.382

0.663

0.294

160 bp–200 bp

30

TUGMS13

F5'GATCTGTGTCTCTCTGTTCCC3'

R5'CCACACATCATCTTTTCCTC3'

(TG)32(TC)24

55°C

7

0.324

0.804

0.675

185 bp–205 bp

25

TUGMS15

F5'GTTGCTTCCTTGGTGCCT3'

R5'GCGGGGACCACATYCAGTA3'

(GA)14

55°C

15

0.500

0.871

0.692

145 bp–190 bp

30

TUGMS17

F5'GGGGAATTTCAGACAGACAC3'

R5'GCCGTTCAGTGTAGTAGATCG3'

(TC)25

55°C

5

0.588

0.796

0.414

160 bp–200 bp

25

TUGMS18

F5'GGGGAAGAAAAAAAAAGTTG3'

R5'TTTCTGGATGTTGTAGTCGG3'

(GA)10

55°C

4

0.059

0.583

0.921

190 bp–260 bp

13

TUGMS20

F5'GGGGAATTTCATCACTCAAAC3'

R5'AGATCGGAGTCACCGTTGTA3'

(TC)24

55°C

4

0.235

0.748

0.727

290 bp–320 bp

21

TUGMS22

F5'GGCAGCTTCAGTTCATCTCT3'

R5'CATAAGGAAAGCTGCAAGAG3'

(GA)13

55°C

8

0.559

0.835

0.626

140 bp–160 bp

24

TUGMS23

F5'GGGGAGCTTACAAAGAGTCA3'

R5'GTGCCGAAGAGAGGATAGAG3'

(TC)13

55°C

8

0.618

0.839

0.503

135 bp–200 bp

31

TUGMS24

F5'CTCACTACAGCRGCAACCGC3'

R5'CCTGAATCTAGTGGGGCTTC3'

(TC)11

55°C

5

0.235

0.727

0.640

280 bp–300 bp

24

TUGMS27***

F5'GGGGATAGTACAAACACACAAC'

R5'GCTCCTCTTTCTTCACCACTT'

(GA)20

55°C

9

-

-

-

80 bp–110 bp

32

TUGMS28

F5' GTCCCCATTGCTCTTAGTTT 3'

R5' GACAATCATTGCCACCACAT 3'

(TG)12

(GA)13

55°C

4

0.529

0.745

0.384

170 bp–200 bp

29

TUGMS29

F5' CAAAACAGAGCCTTCATAAG 3'

R5' ATCGAGACAGAAGACAGACG 3'

(TC)20

53°C

4

0.029

0.481

0.968

105 bp–120 bp

10

TUGMS31B

F5' CTATGTACGACTCTCTGCCTG3'

R5' GTTTGTCTGGAGTTAAACGAG3'

(TC)9

55°C

5

0.294

0.558

0.853

140 bp–170 bp

12

TUGMS33

F5'CCCTCTTCTCTCACCAGATC3'

R5'TCCCTTCTTTGCCTTCTACA3'

(GA)10

55°C

3

0.441

0.615

0.180

150 bp–160 bp

34

TUGMS34

F5'GCCAAAATTCCATCTAGGG3'

R5'TGCAACTCGTATGTGGACC3'

(TTC)18(GA)10

55°C

10

0.618

0.828

0.364

160 bp–200 bp

32

TUGMS35

F5'GGGGCTCTCTCTCTCTAAAG3'

R5'TGCTGTGAGAAGTAAAGGGC3'

(TC)11

55°C

6

0.853

0.848

0.267

110 bp–140 bp

30

TUGMS36

F5'GCCAGCAAGTAAGAGAAGCT3'

R5'GTGGGTTGAGTACCAACAGG3'

(GA)13

55°C

2

0.029

0.510

0.856

125 bp–115 bp

13

TUGMS41

F5'CCTTTCACAACAGATCCACA3'

R5'GAGCTTCCTGACGATGGTTA3'

(GA)11

55°C

3

0.235

0.625

0.717

115 bp–120 bp

16

TUGMS42

F5'GTAGCTCGCAACACAACACC3'

R5'CTCCAACGACACACTCTCTG3'

(GA)23

(GA)11

55°C

7

0.588

0.860

0.581

100 bp–180 bp

29

TUGMS43A

F5'CATTTCCTTCTCACCCCTAC3'

R5'GTGGGTGTGGGACTTGAATA3'

(GA)11

55°C

4

0.264

0.576

0.842

150 bp–170 bp

13

TUGMS44

F5'GTGTTGGGAGTGTTGCTGAA3'

R5'ACCACCTGATTCGACATCTC3'

(GA)12

55°C

4

0.118

0.478

0.353

300 bp–360 bp

25

TUGMS45

F5'GGGGATTGTTGAAGTTTCTC3'

R5'CTTCACCCATATCTTCCAAA3'

(GA)20

55°C

2

0.118

0.555

0.764

148 bp–150 bp

16

TUGMS46***

F5'GGGTTCAGTCGCAGCAAA3'

R5'GAGGAGTTCTTCTTGCGTCT3'

(TC)13

55°C

10

-

-

-

98 bp–120 bp

34

TUGMS48

F5'TCGGGCAACCACCATATATA3'

R5'CTTTTCCCACCAGACAAGAA3'

(GA)14

55°C

7

0.441

0.880

0.755

100 bp–135 bp

28

TUGMS50

F5'GGGGATTCATCTCTGAACAC3'

R5'GGAGAGAGTGAGAGCTTTGG3'

(TC)11

55°C

2

0.059

0.527

0.932

168 bp–175 bp

12

TUGMS51

F5'CCAGACTCATCGCAGAAATC3'

R5'GGTTGGGTGAGGAGGAATAG3'

(GA)11

55°C

7

0.765

0.792

0.353

145 bp–170 bp

32

TUGMS52

F5'GAACCAACCCAGTCTATACTCC3'

R5'AGCACACGCCATCCAATC3'

(GA)14

55°C

16

0.794

0.909

0.622

90 bp–120 bp

34

TUGMS58A

F5'TTCTTCCTCTTCTTTGGTGG3'

R5'AGAGGGTGAAGAGGAAGTTG3'

(TCC)14

55°C

4

0.647

0.678

0.210

90 bp–110 bp

31

TUGMS58B

F5'CAACTTCCTCTTCACCCTCT3'

R5'GCTGAAGAGAACGGTGAAGA3'

(TCG)28

55°C

2

0.059

0.216

0.124

140 bp–160 bp

31

TUGMS59

F5'CACCTTCATCTTCACCTTCC3'

R5'TGAGTCTGCTCGTAGGTGAG3'

(TGG)9

55°C

3

0.382

0.454

0.018

168 bp–180 bp

25

TUGMS63

F5'CAAGGTAAAGGACATGCACC3'

R5'GTCCTCAGAAGCCATCGAA3'

(CCG)6

55°C

2

0.177

0.613

0.568

150 bp–155 bp

22

TUGMS64

F5'TGCAGGGGAGATGAATTAAC3'

R5'ACCTGCATTTCCCAGTCTT3'

(CAG)9

55°C

5

0.382

0.775

0.605

280 bp–320 bp

24

TUGMS66

F5'AATGGTTGGGTAAGCCTCT3'

R5'TGACCAACAACGGATCACA3'

(TTA)8

55°C

4

0.441

0.644

0.231

220 bp–320 bp

29

TUGMS70

F5'ATCAGACGATGTACCGAAGAG3'

R5'CGAACGTGAATGTAATCAGG3'

(CAA)15

55°C

2

0.029

0.504

0.782

180 bp–190 bp

14

TUGMS71

F5'AGCAGCAAGTGTCGTTTACA3'

R5'GCAGAAATGAGAGAAGGAGG3'

(GAA)8

55°C

3

0.235

0.511

0.204

240 bp–320 bp

30

TUGMS72

F5'CCAGCTCGATAGCATCTACA3'

R5'CACTATCCAAATCCATCGC3'

(ATG)10

55°C

2

0.559

0.396

0.072

198 bp–205 bp

28

TUGMS73

F5'GTCAAGACGCCCACTACAGT3'

R5'GACTGTGTAACCTGCCAAGAC3'

(TAA)12

55°C

9

0.677

0.907

0.694

150 bp–220 bp

32

TUGMS74

F5'CACCCCCTTCCTATTCAAA3'

R5'AGGTGGTCACTTCTTCAACG3'

(CCA)9

55°C

6

0.706

0.900

0.676

170 bp–200 bp

32

TUGMS75

F5'GGTGATCCGATGGTGAATT3'

R5'ACAGGAGCATCAACAGCAGG3'

(ATG)9

55°C

4

0.265

0.293

0.032

240 bp–280 bp

34

TUGMS76

F5'AGATGAGCACAAGGAAGGAG3'

R5'CGAAGTAGTGTAGGGGAAGAA3'

(GAA)10

55°C

2

0.441

0.618

0.652

198 bp–210 bp

16

TUGMS77

F5'CTACCCTTCTTCTCAGTTCCA3'

R5'CAGATGAAATGAAGGGCATC3'

(CAA)10

55°C

2

0.206

0.490

0.848

132 bp–140 bp

11

TUGMS78

F5'CACCGCTTGACTAAAATGG3'

R5'AAACTATCAACCGTATGGGC3'

(TTC)13

55°C

8

0.647

0.878

0.597

130 bp–170 bp

31

TUGMS79

F5'GGGTAATTTAAGGGTGTCCT3'

R5'AAGAGGGTGATAAGGATTCC3'

(CCA)11

55°C

7

0.324

0.682

0.503

160 bp–260 bp

24

TUGMS82

F5'AAGTTAGAGAGAGAGAAGTGGC3'

R5'AATGCCACACCAGTCCTAG3'

(CAT)8

55°C

6

0.412

0.691

0.344

140 bp–180 bp

30

TUGMS83

F5'GAGGATTTGGGTTTGTGAAC3'

R5'TCATTCTCTCTGGCATCACC3'

(TGG)9

55°C

4

0.765

0.671

0.242

250 bp–600 bp

30

TUGMS84

F5'GCTAGGCATTCGAGGAGTT3'

R5'GGACTCCTCACTGCTTGAAG3'

(ATG)34

55°C

2

0.500

0.499

0.060

220 bp–500 bp

31

TUGMS85

F5'GACGGAAAATCGAAGGC3'

R5'TCTTACTGCTCTTGGCTTCC3'

(GAA)11

55°C

3

0.059

0.140

0.063

120 bp–140 bp

34

TUGMS87

F5'TCACATTTTCAGAGGAAGAGG3'

R5'TTAGGGTTTAGTTGTGGCTG3'

(GAA)6

55°C

5

0.235

0.733

0.673

90 bp–125 bp

28

TUGMS90

F5'GAGGGGAAGTGTGAAAAATC3'

R5'TTGGGATTCCTTTTCTATGC3'

(TTTG)6

55°C

4

0.412

0.696

0.607

95 bp–120 bp

18

TUGMS92

F5'TCTATCAGTTGGCTTGGTTG3'

R5'CAATCTTTCACTGGCATGAG3'

(TTTC)13

55°C

4

0.118

0.269

0.972

145 bp–180 bp

5

TUGMS95

F55'GGCTCCTTCCTCTTCTGATC3'

R5'TGAAGTTGGGATTGAGCATG3'

(CAAA)6

55°C

5

0.412

0.777

0.596

120 bp–150 bp

23

TUGMS98

F5'AGCCCAACTCCTCCTGAC3'

R55'GAGCAGCCTCATTCGGAC3'

(TTGTG)8

55°C

3

0.441

0.613

0.223

280 bp–380 bp

31

TUGMS99

F5'GAATAGGGTTTGGCAGAGGC3'

R5'AGGATGGAGGAGGTGTCAA3'

(GGGAGA)7

(GAGAA)6

55°C

6

0.206

0.154

0.542

160 bp–200 bp

25

TUGMS102A

F5'CGTAGCTCGCACACAACAC3'

R5'CGTCCCCTCCGAAATGA3'

(GGAAA)12

55°C

6

0.706

0.803

0.230

100 bp–120 bp

27

TUGMS105

F5'GGGAGCTAGGGTTTTAGTTT3'

R5'CTTCAGAGCCACTTCTTTGTC3'

(TTCTC)5

55°C

5

0.618

0.711

0.098

150 bp–200 bp

30

TUGMS108

F5'GGGACATCATCACCAGCTT3'

R5'TTCCTTGGTAGAACTCTGCTT3'

(CAAAAA)6

55°C

6

0.853

0.790

0.141

130 bp–160 bp

30

  1. TUGMS,Tea unigene microsatellite; bp, base pair; H O , Observed heterozygosities; H E , Expected heterozygosities)
  2. * Accession details of contributing ESTs for unigenes are given in additional file (see Additional file 1).
  3. **A significant deviation in Hardy-Weinberg equilibrium at P < 0.001 level recorded all single locus UGMS primers.
  4. ***TGUMS marker with multi locus amplifications.
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BMC Plant Biology

ISSN: 1471-2229

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