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Figure 2 | BMC Plant Biology

Figure 2

From: Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Figure 2

Growth characteristics of controls and mutant plants complemented with promoter:: cSUC2 cassettes. A, Rosette area (cm2) of 21-day old wild type (AtSUC2 +/+), heterozygous (AtSUC2 +/-), homozygous mutant (AtSUC2 -/-), and homozygous mutant plants independently transformed (indicated by seed-stock number) with the indicated cSUC2 constructs; n = 4 to 10 sibling plants. Lines marked * are homozygous for the promoter::cSUC2 cassettes, and from these, a representative line for each was chosen for further analysis. B to D, representative 21-day old AtSUC2 +/+, AtSUC2 +/-, and AtSUC2 -/- plants, respectively. E to H, Representative 21-day old AtSUC2 -/- plants transformed with SUC2p::cSUC2::uidA (E; line 1042) SUC2p::cSUC2 (F; line 1039), CoYMVp::cSUC2 (G; line 1070), and rolCp::cSUC2 (H; line 1133) constructs. Scale bar, B through H = 1 cm. I to L, XGlcA staining in source leaves of untransformed wild type (I), and heterozygous AtSUC2 +/- plants transformed with SUC2p::cSUC2::uidA (J), CoYMVp::cSUC2::uidA (K), and rolCp::cSUC2::uidA (L). The staining pattern was the same irrespective of zygosity at the AtSUC2 locus. Scale bar, I through L = 1 mm.

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