Figure 1

Functional comparison of the most common deleterious FRI alleles. (a) Leaf number at flowering from individuals in the Col x Ler recombinant inbred line set grouped by their genotype at FRI and FLC. (b) Vernalization response of Arabidopsis natural accessions measured as the reduction of days to flowering in plants vernalized for 5 weeks at 4°C compared to unvernalized plants. Accessions are grouped by their FRI alleles as described in [22]. The number at the bottom of each bar indicates the number of accessions in each group; error bars in (a) and (b) correspond to the standard error of the mean. Letters in each bar or point represent significance groups as determined by Tukey HSD test. (c) Exonic organization for FRI-wt (Bil-7), FRI-Col and FRI-Ler. Red regions indicate the indels characteristic for FRI-Col and FRI-Ler. (d) Predicted proteins for all three alleles shown in (c). The dashed region in FRI-Col represents a shift in the ORF caused by the 16 bp deletion. Shaded regions in the background indicate predicted coiled-coil domains.