Figure 5

Immunolocalization of non-esterified (JIM5) and highly-esterified (JIM7) pectins at early in vitro embryogenesis of microspore and immature zygotic embryos. A, A’, C, C’, E, E’, G, G’: Microspore embryogenesis. B, B’, D, D’, F, F’, H, H’: Somatic embryogenesis of immature zygotic embryos. A’-H’: Confocal merged images of immunofluorescence signal (green) of JIM7 (A’-D’) or JIM5 (E’-H’) combined with DAPI staining of nuclei (blue). A-H: Differential interference contrast, DIC, images of the same sections. A, A’, B, B’, E, E’, F, F’: Early multicellular embryos. C, C’, D, D’, G, G’, H, H’: Large multicellular and globular embryos (emb) emerging from embryogenic masses (ms) by secondary embryogenesis. Bars: 100 μm.