Figure 5

Transcript abundance of PtTPS6 and PtTPS9 genes in individual poplar leaves. Transcript abundance of TPS genes were measured in either control trees (ctr), trees which received herbivory apically on leaf LPI3 (ha, herbivory apical) or basally on leaf LPI10 (hb, herbivory basal). QRT-PCR was performed for herbivore-treated leaves (LPI3 and LPI10), their closest neighboring leaves (LPI4 and LPI9) and vascularly connected leaves (LPI8 and LPI5). Means and standard errors are shown (n = 5). The student's t-test was used to test for statistical significance between the two treatments in one leaf. Asterisks indicate significant differences (p < 0.001, ** p < 0.01, * p < 0.05).