Figure 5From: Tomato SR/CAMTA transcription factors SlSR1 and SlSR3L negatively regulate disease resistance response and SlSR1L positively modulates drought stress toleranceSilencing of either SlSR1 or SlSR3L led to enhanced resistance to P. syringae pv. tomato DC3000. Two-week-old tomato seedlings were infiltrated with agrobacteria carrying pTRV2-SlSRs or pTRV2-GUS constructs and the pTRV2-GUS- and pTRV2-SlSRs-infiltrated plants were inoculated by vacuum infiltration with infiltration with P. syringae pv. tomato DC3000 (OD600?=?0.0002) at 3 weeks after VIGS infiltration. (A) Representative symptom of disease caused by P. syringae pv. tomato DC3000 at 4 days after inoculation. (B) Bacterial growth in inoculated leaves of pTRV2-GUS- and pTRV2-SlSRs-infiltrated plants. Leaf samples were collected at 0 and 4 days after inoculation and bacterial growth was measured. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p?<?0.05 level.Back to article page