Figure 2

Molecular analysis in transgenic soybean plants. PCR products of hptII and SnOLP genes amplified from DNA extracted from transgenic plants and controls. Detection of the SnOLP protein (~27 kDa) in soybean plants by Western blot hybridization. +: vector pCL1390-UBQ3-SnOLP (PCR positive control). B1, B2 and B3 lines: plants recovered from three putative transformation events. B1 line was represented by 10 clone plants. B2 and B3 lines were represented by one plant each. Two Bragg wild-type (WT) plants were used as negative controls.