Figure 1

De novo expression of the turtle RfBP gene and its effects on flowering and expression of FD and AP1 genes in Arabidopsis. WT, RfBP⁺, and RfBP⁻ plants were grown in long days. Northern blotting (a) and electrophoresis (b) analyses were performed with RNAs and proteins, respectively, isolated from the two youngest expanded leaves of 12-day-old plants. Gel staining with in (b) verified consistent loading of proteins. Three-week-old plants were photographed (c). Days to flower and rosette leaf number were scored as mean values ± standard deviations from seven experimental repeats each containing 50 plants (d). On bar graphs, different letters shown in regular and italic fonts indicate significant differences by analysis of variance using Fisher’s least significant difference test and Tukey-Kramer’s test, respectively (n = 7; P < 0.01). FD and AP1 were analyzed by Northern blotting with RNAs from shoot apices of 12-day-old plants (e). In (a) and (e), the constitutively expressed EF1α gene was used as a reference.