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Table 1 Changes in average normalized spot volumes of flour proteins in transgenic lines relative to the non-transgenic control grown with and without post-anthesis fertilizer

From: Silencing of omega-5 gliadins in transgenic wheat eliminates a major source of environmental variability and improves dough mixing properties of flour

   # 2-DE spots that decrease 1 # 2-DE spots that increase 1 Average spot volume 2 % Change 3
   Minus fertilizer Plus fertilizer Minus fertilizer Plus fertilizer Minus fertilizer Plus fertilizer Minus fertilizer Plus fertilizer
# Spots 4 Protein Identification 5 35b 45a 35b 45a 35b 45a 35b 45a Control 35b 45a Control 35b 45a 35b 45a 35b 45a
8 HMW-GS Ax2*          478.37 478.88 501.45 561.18 557.40 570.27 0.1 4.8 −0.7 1.6
8 HMW-GS Bx7          824.00 850.20 871.81 968.99 987.02 938.06 3.2 5.8 1.9 −3.2
8 HMW-GS By9       3   1 417.26 431.28 505.14 550.88 583.14 635.55 3.4 21.1 5.9 15.4
8 HMW-GS Dx5   3 2 4      755.94 722.52 652.04 848.64 822.21 684.83 −4.4 −13.7 −3.1 −19.3
7 HMW-GS Dy10       1   1 594.53 597.64 650.34 702.68 707.96 751.93 0.5 9.4 0.8 7.0
39 Total HMW-GS          3070.10 3080.52 3180.79 3632.37 3657.73 3580.64 0.3 3.6 0.7 −1.4
2 LMW-GS Bu-1 (m-type)6          625.10 600.98 646.79 647.11 629.87 653.32 −3.9 3.5 −2.7 1.0
4 LMW-GS Bu-2/13 (s-type)6     2      551.57 552.98 493.66 638.08 640.46 532.08 0.3 −10.5 0.4 −16.6
8 LMW-GS Bu-3 (s-type)6 1 4 1 3      1266.56 1275.58 1061.02 1264.48 1249.93 1048.88 0.7 −16.2 −1.2 −17.1
3 LMW-GS Bu-4 (i-type)6          353.30 380.21 403.80 377.56 380.24 405.58 7.6 4.3 0.7 7.4
1 LMW-GS Bu-6 (m-type)6          254.17 253.36 249.13 226.14 214.33 208.26 −0.3 −2.0 −5.2 −7.9
3 LMW-GS Bu-7 (m-type)6    1       523.29 534.42 572.33 499.02 497.83 521.43 2.1 9.4 −0.2 4.5
1 LMW-GS Bu-8 (m-type)6          32.70 31.85 28.07 37.40 31.53 37.11 −2.6 −14.2 −15.7 −0.8
1 LMW-GS Bu-11 (m-type)6          124.72 132.19 140.70 107.97 108.53 110.90 6.0 2.8 0.5 2.7
2 LMW-GS Bu-18 (m-type)6          270.35 293.55 326.37 255.90 265.262 56.40 8.6 20.7 3.7 0.2
1 LMW-GS TC11-277270 (m-type)6   1   1      150.06 150.51 149.04 149.07 167.17 138.69 0.3 −0.7 12.1 −7.0
1 LMW-GS [GenBank: AAB48469] (i-type)6          60.73 54.40 34.03 68.93 67.85 40.05 −10.4 44.0 −1.6 −41.9
27 Total traditional LMW-GS          4212.57 4260.01 4104.93 4271.68 4253.01 3952.71 1.1 −2.6 −0.4 −7.5
2 Alpha chain-terminating gliadin Bu-27       1   1 402.61 410.38 451.61 451.36 455.88 485.50 1.9 12.2 1.0 7.6
3 Gamma chain-terminating gliadin Bu-48   2 1 1      476.24 481.89 415.65 473.78 478.97 430.01 1.2 −12.7 1.1 −9.2
4 Omega-1,2 chain-terminating gliadin9   3   4      283.38 277.98 195.36 536.24 537.38 246.83 −1.9 −31.1 0.2 −54.0
9 Total chain-terminating gliadins          1162.23 1170.26 1062.62 1461.38 1472.22 1162.33 0.7 −8.6 0.7 −20.5
1 Alpha gliadin Bu-1          68.09 68.56 74.33 81.37 69.73 85.50 0.7 9.2 −14.3 5.1
1 Alpha gliadin Bu-3          180.74 182.36 204.19 210.70 221.22 232.06 0.9 13.0 5.0 10.1
1 Alpha gliadin Bu-4          306.35 313.67 349.35 353.82 354.76 367.54 2.4 4.0 0.3 3.9
2 Alpha gliadin Bu-5       1    190.87 209.23 239.66 285.97 296.50 312.78 9.6 25.6 3.7 9.4
1 Alpha gliadin Bu-10          196.26 206.54 197.39 208.34 214.01 201.55 5.2 0.6 2.7 −3.3
1 Alpha gliadin Bu-11          205.24 212.00 245.84 272.06 279.76 303.77 3.3 19.8 2.8 11.7
4 Alpha gliadin Bu-12       1    506.20 511.08 617.13 698.95 707.77 784.63 1.0 21.9 1.3 12.3
4 Alpha gliadin Bu-14       2 1 3 271.13 289.98 348.01 358.25 384.61 446.39 7.0 28.4 7.4 24.6
1 Alpha gliadin Bu-23          290.26 297.86 277.91 336.09 340.86 330.65 2.6 −4.3 1.4 −1.6
1 Alpha gliadin Bu-27 1   1    1    114.68 50.80 155.69 142.19 41.25 169.12 −55.7 35.8 −71.0 18.9
1 Alpha gliadin Bu-BQ806209          80.78 81.85 73.91 74.03 66.24 3.0 4.4 0.2 −10.4 80.78
1 Alpha gliadin Bu-BQ807130          96.61 107.05 114.95 125.15 119.48 135.56 10.8 19.0 −4.5 8.3
19 Total alpha gliadin          2504.83 2529.92 2906.30 3146.79 3103.98 3435.79 1.0 16.0 −1.4 9.2
2 Gamma gliadin Bu-1   1   2      205.88 217.38 179.18 223.09 212.75 176.47 5.6 −13.0 −4.6 −20.9
1 Gamma gliadin Bu-2          298.32 306.62 266.99 337.45 332.72 295.21 2.8 −10.5 −1.4 −12.5
4 Gamma gliadin Bu-5   1        795.04 816.60 738.76 798.55 802.41 719.11 2.7 −7.1 0.5 −9.9
3 Gamma gliadin Bu-6     2      294.02 308.72 291.19 341.59 321.44 282.37 5.0 −1.0 −5.9 −17.3
1 Gamma gliadin Bu-7          109.93 115.62 119.32 117.19 110.12 126.22 5.2 8.5 −6.0 7.7
2 Gamma gliadin Bu-11          281.31 285.23 300.08 303.39 296.76 315.99 1.4 6.7 −2.2 4.2
13 Total gamma gliadin          1984.50 2050.18 1895.51 2121.27 2076.21 1915.37 3.3 −4.5 −2.1 −9.7
6 Omega-5 gliadin 6 6 6 6      675.82 229.83 146.27 1153.93 237.46 140.56 −66.0 −78.4 −79.4 −87.8
2 Omega-1,2 gliadin D310   1   2      479.80 458.24 393.76 631.49 581.38 410.86 −4.5 −17.9 −7.9 −34.9
2 Omega-1,2 gliadin Bu-D510 1 2 1 2      242.54 214.06 111.87 435.25 355.39 133.28 −11.7 −53.9 −18.3 −69.4
1 Secalin-like omega gliadin          37.73 41.88 49.08 49.63 52.13 56.00 11.0 30.1 5.1 12.8
11 Total omega gliadin          1435.89 944.01 700.98 2270.30 1226.37 740.69 −34.3 −51.2 −46.0 −67.4
4 Total mixed gliadin          404.96 425.58 424.86 432.88 460.34 472.80 51 4.9 6.3 9.2
7 Total triticin       1   3 673.65 727.12 794.69 777.11 839.56 964.18 7.9 18.0 8.0 24.1
1 Farinin Bu-1          58.89 55.48 68.66 41.31 33.45 39.08 −5.8 16.6 −19.0 −5.4
3 Farinin Bu-2          334.76 320.74 307.56 231.53 221.26 221.18 −4.2 −8.1 −4.4 −4.5
2 Farinin Bu-3          143.03 154.74 146.48 125.87 124.27 128.67 8.2 2.4 −1.3 2.2
6 Total farinin          536.68 530.95 522.70 398.71 378.98 388.94 −1.1 2.6 −4.9 2.5
2 Purinin Bu-1       1    204.59 217.28 239.48 180.15 162.16 175.04 6.2 17.1 −10.0 −2.8
2 Purinin Bu-2     1      213.30 224.44 247.53 185.90 174.15 168.17 5.2 16.0 −6.3 −9.5
2 Purinin Bu-3          457.02 475.77 529.57 423.85 423.25 400.29 4.1 15.9 −0.1 −5.6
6 Total purinin          874.92 917.50 1016.58 789.89 759.56 743.49 4.9 16.2 −3.8 −5.9
1 Globulin-1          41.35 41.57 52.98 58.73 58.46 70.51 0.5 28.1 −0.5 20.1
5 Globulin-2 1 2 2 2      115.61 105.93 102.16 104.84 98.49 91.10 −8.4 −11.6 −6.1 −13.1
4 Globulin Glo-3   1   1      246.03 257.17 215.45 210.61 225.19 187.46 4.5 −12.4 6.9 −11.0
10 Total globulin          402.98 404.67 370.59 374.18 382.14 349.08 0.4 −8.0 2.1 −6.7
4 Serpin Bu-1       3    168.10 162.31 211.52 251.48 253.04 278.30 −3.4 25.8 0.6 10.7
2 Serpin Bu-2       2    53.48 52.28 70.47 108.94 114.09 119.34 −2.2 31.8 4.7 9.6
2 Serpin Bu-3         1 41.23 40.67 50.63 63.64 66.61 81.15 −1.4 22.8 4.7 27.5
1 Serpin Bu-4       1    67.65 69.35 83.86 105.73 111.12 128.48 2.5 24.0 5.1 21.5
2 Serpin Bu-5          83.63 83.32 103.75 126.05 125.89 152.76 −0.4 24.1 −0.1 21.2
2 Serpin Bu-7 1 1 1 1 1 1 1 1 59.27 62.64 63.17 74.88 70.10 73.42 5.7 6.6 −6.4 −2.0
13 Total serpin          473.36 470.56 583.41 730.71 740.86 833.45 −0.6 23.2 1.4 14.1
2 Beta-amylase Bu-2          102.36 93.67 101.60 99.71 98.17 99.37 −8.5 −0.7 −1.5 −0.3
3 Beta-amylase Bu-3 1 2 3 1      117.39 99.37 100.84 136.55 87.79 98.43 −15.4 −14.1 −35.7 −27.9
5 Total beta-amylase          219.75 193.03 202.43 236.27 185.97 197.80 −12.2 −7.9 −21.3 −16.3
2 WMAI Bu-1          624.43 660.27 661.82 469.28 488.91 456.32 5.7 6.0 4.2 −2.8
1 WDAI Bu-1          616.66 621.14 659.08 506.27 464.64 489.27 0.7 6.9 −8.2 −3.4
1 WDAI Bu-3          256.36 259.57 294.40 245.10 230.20 273.67 1.3 14.8 −6.1 11.7
1 WDAI Bu-4          179.79 194.48 206.86 130.61 113.52 126.49 8.2 15.1 −13.1 −3.1
1 WTAI CM1          223.52 233.25 247.31 183.44 172.34 170.34 4.3 10.6 −6.1 −7.1
2 WTAI CM2    1       1028.76 1046.46 1023.65 760.84 682.30 707.44 1.7 −0.5 −10.3 −7.0
2 WTAI CM3          653.13 660.93 658.76 483.08 436.22 402.44 1.2 0.9 −9.7 −16.7
2 WTAI CM16    1 1      549.43 517.83 535.74 397.30 332.88 337.40 −5.8 −2.5 −16.2 −15.1
2 WTAI CM17          442.72 437.00 443.39 307.31 263.48 266.70 −1.3 0.1 −14.3 −13.2
2 WCI          402.00 420.67 403.68 281.27 260.35 243.91 4.6 0.4 −7.4 −13.3
2 CMX1/CMX3          256.24 268.07 268.12 197.36 191.19 184.98 4.6 4.6 −3.1 −6.3
1 WASI          250.63 248.99 262.19 177.70 184.67 188.80 −0.7 4.6 3.9 6.2
19 Total amylase/protease inhibitors          5483.67 5568.66 5664.99 4139.56 3820.69 3847.76 1.5 3.3 −7.7 −7.0
32 Other identified proteins   1   1 1     2056.00 2108.86 2064.67 1888.87 1871.32 1966.62 2.6 0.4 −0.9 4.1
  1. 1Number of spots identified as each protein type that increased or decreased at least 20% in transgenic lines relative to non-transgenic control. Spot numbers and identities of all 2-DE spots that change can be found in Additional file 3.
  2. 2Sum of average volumes of all spots with the same MS/MS identification. MS/MS identifications are from [6].
  3. 3Protein types that that increased or decreased at least 20% in transgenic lines relative to non-transgenic control are indicated in bold. Changes that were greater than 20% but were not deemed to be significant by ANOVA are shown in bold italics.
  4. 4Total number of spots in 2-D gels that were identified as the same protein type in [6].
  5. 5Predominant protein in spots identified by MS/MS in [6]. Protein classes are indicated in bold type.
  6. 6Classification of traditional LMW-GS as m-type, s-type and i-type is based on the first amino acid of the mature proteins; methionine, serine or isoleucine.
  7. 7Protein has amino acid sequence similar to an alpha gliadin but contains seven cysteines that enables it to be linked into the glutenin polymer.
  8. 8Protein has amino acid sequence similar to a gamma gliadin but contains nine cysteines that enables it to be linked into the glutenin polymer.
  9. 9Protein has amino acid sequence similar to an omega-1,2 gliadin but contains one cysteine that enables it to be linked into the glutenin polymer.
  10. 10Two distinct protein types were distinguished for omega-1,2 gliadins. Percent changes of total omega-1,2 gliadins are −6.9 and −30.0 for 35b and 45a grown without fertilizer, respectively, and −12.2 and −49.0 for 35b and 45a grown with fertilizer, respectively.