|  | # 2-DE spots that decrease 1 | # 2-DE spots that increase 1 | Average spot volume 2 | % Change 3 | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 |  | Minus fertilizer | Plus fertilizer | Minus fertilizer | Plus fertilizer | Minus fertilizer | Plus fertilizer | Minus fertilizer | Plus fertilizer | ||||||||||
# Spots 4 | Protein Identification 5 | 35b | 45a | 35b | 45a | 35b | 45a | 35b | 45a | Control | 35b | 45a | Control | 35b | 45a | 35b | 45a | 35b | 45a |
8 | HMW-GS Ax2* |  |  |  |  |  |  |  |  | 478.37 | 478.88 | 501.45 | 561.18 | 557.40 | 570.27 | 0.1 | 4.8 | −0.7 | 1.6 |
8 | HMW-GS Bx7 |  |  |  |  |  |  |  |  | 824.00 | 850.20 | 871.81 | 968.99 | 987.02 | 938.06 | 3.2 | 5.8 | 1.9 | −3.2 |
8 | HMW-GS By9 | Â | Â | Â | Â | Â | 3 | Â | 1 | 417.26 | 431.28 | 505.14 | 550.88 | 583.14 | 635.55 | 3.4 | 21.1 | 5.9 | 15.4 |
8 | HMW-GS Dx5 |  | 3 | 2 | 4 |  |  |  |  | 755.94 | 722.52 | 652.04 | 848.64 | 822.21 | 684.83 | −4.4 | −13.7 | −3.1 | −19.3 |
7 | HMW-GS Dy10 | Â | Â | Â | Â | Â | 1 | Â | 1 | 594.53 | 597.64 | 650.34 | 702.68 | 707.96 | 751.93 | 0.5 | 9.4 | 0.8 | 7.0 |
39 | Total HMW-GS |  |  |  |  |  |  |  |  | 3070.10 | 3080.52 | 3180.79 | 3632.37 | 3657.73 | 3580.64 | 0.3 | 3.6 | 0.7 | −1.4 |
2 | LMW-GS Bu-1 (m-type)6 |  |  |  |  |  |  |  |  | 625.10 | 600.98 | 646.79 | 647.11 | 629.87 | 653.32 | −3.9 | 3.5 | −2.7 | 1.0 |
4 | LMW-GS Bu-2/13 (s-type)6 |  |  |  | 2 |  |  |  |  | 551.57 | 552.98 | 493.66 | 638.08 | 640.46 | 532.08 | 0.3 | −10.5 | 0.4 | −16.6 |
8 | LMW-GS Bu-3 (s-type)6 | 1 | 4 | 1 | 3 |  |  |  |  | 1266.56 | 1275.58 | 1061.02 | 1264.48 | 1249.93 | 1048.88 | 0.7 | −16.2 | −1.2 | −17.1 |
3 | LMW-GS Bu-4 (i-type)6 | Â | Â | Â | Â | Â | Â | Â | Â | 353.30 | 380.21 | 403.80 | 377.56 | 380.24 | 405.58 | 7.6 | 4.3 | 0.7 | 7.4 |
1 | LMW-GS Bu-6 (m-type)6 |  |  |  |  |  |  |  |  | 254.17 | 253.36 | 249.13 | 226.14 | 214.33 | 208.26 | −0.3 | −2.0 | −5.2 | −7.9 |
3 | LMW-GS Bu-7 (m-type)6 |  |  | 1 |  |  |  |  |  | 523.29 | 534.42 | 572.33 | 499.02 | 497.83 | 521.43 | 2.1 | 9.4 | −0.2 | 4.5 |
1 | LMW-GS Bu-8 (m-type)6 |  |  |  |  |  |  |  |  | 32.70 | 31.85 | 28.07 | 37.40 | 31.53 | 37.11 | −2.6 | −14.2 | −15.7 | −0.8 |
1 | LMW-GS Bu-11 (m-type)6 | Â | Â | Â | Â | Â | Â | Â | Â | 124.72 | 132.19 | 140.70 | 107.97 | 108.53 | 110.90 | 6.0 | 2.8 | 0.5 | 2.7 |
2 | LMW-GS Bu-18 (m-type)6 | Â | Â | Â | Â | Â | Â | Â | Â | 270.35 | 293.55 | 326.37 | 255.90 | 265.262 | 56.40 | 8.6 | 20.7 | 3.7 | 0.2 |
1 | LMW-GS TC11-277270 (m-type)6 |  | 1 |  | 1 |  |  |  |  | 150.06 | 150.51 | 149.04 | 149.07 | 167.17 | 138.69 | 0.3 | −0.7 | 12.1 | −7.0 |
1 | LMW-GS [GenBank: AAB48469] (i-type)6 |  |  |  |  |  |  |  |  | 60.73 | 54.40 | 34.03 | 68.93 | 67.85 | 40.05 | −10.4 | −44.0 | −1.6 | −41.9 |
27 | Total traditional LMW-GS |  |  |  |  |  |  |  |  | 4212.57 | 4260.01 | 4104.93 | 4271.68 | 4253.01 | 3952.71 | 1.1 | −2.6 | −0.4 | −7.5 |
2 | Alpha chain-terminating gliadin Bu-27 | Â | Â | Â | Â | Â | 1 | Â | 1 | 402.61 | 410.38 | 451.61 | 451.36 | 455.88 | 485.50 | 1.9 | 12.2 | 1.0 | 7.6 |
3 | Gamma chain-terminating gliadin Bu-48 |  | 2 | 1 | 1 |  |  |  |  | 476.24 | 481.89 | 415.65 | 473.78 | 478.97 | 430.01 | 1.2 | −12.7 | 1.1 | −9.2 |
4 | Omega-1,2 chain-terminating gliadin9 |  | 3 |  | 4 |  |  |  |  | 283.38 | 277.98 | 195.36 | 536.24 | 537.38 | 246.83 | −1.9 | −31.1 | 0.2 | −54.0 |
9 | Total chain-terminating gliadins |  |  |  |  |  |  |  |  | 1162.23 | 1170.26 | 1062.62 | 1461.38 | 1472.22 | 1162.33 | 0.7 | −8.6 | 0.7 | −20.5 |
1 | Alpha gliadin Bu-1 |  |  |  |  |  |  |  |  | 68.09 | 68.56 | 74.33 | 81.37 | 69.73 | 85.50 | 0.7 | 9.2 | −14.3 | 5.1 |
1 | Alpha gliadin Bu-3 | Â | Â | Â | Â | Â | Â | Â | Â | 180.74 | 182.36 | 204.19 | 210.70 | 221.22 | 232.06 | 0.9 | 13.0 | 5.0 | 10.1 |
1 | Alpha gliadin Bu-4 | Â | Â | Â | Â | Â | Â | Â | Â | 306.35 | 313.67 | 349.35 | 353.82 | 354.76 | 367.54 | 2.4 | 4.0 | 0.3 | 3.9 |
2 | Alpha gliadin Bu-5 | Â | Â | Â | Â | Â | 1 | Â | Â | 190.87 | 209.23 | 239.66 | 285.97 | 296.50 | 312.78 | 9.6 | 25.6 | 3.7 | 9.4 |
1 | Alpha gliadin Bu-10 |  |  |  |  |  |  |  |  | 196.26 | 206.54 | 197.39 | 208.34 | 214.01 | 201.55 | 5.2 | 0.6 | 2.7 | −3.3 |
1 | Alpha gliadin Bu-11 | Â | Â | Â | Â | Â | Â | Â | Â | 205.24 | 212.00 | 245.84 | 272.06 | 279.76 | 303.77 | 3.3 | 19.8 | 2.8 | 11.7 |
4 | Alpha gliadin Bu-12 | Â | Â | Â | Â | Â | 1 | Â | Â | 506.20 | 511.08 | 617.13 | 698.95 | 707.77 | 784.63 | 1.0 | 21.9 | 1.3 | 12.3 |
4 | Alpha gliadin Bu-14 | Â | Â | Â | Â | Â | 2 | 1 | 3 | 271.13 | 289.98 | 348.01 | 358.25 | 384.61 | 446.39 | 7.0 | 28.4 | 7.4 | 24.6 |
1 | Alpha gliadin Bu-23 |  |  |  |  |  |  |  |  | 290.26 | 297.86 | 277.91 | 336.09 | 340.86 | 330.65 | 2.6 | −4.3 | 1.4 | −1.6 |
1 | Alpha gliadin Bu-27 | 1 |  | 1 |  |  | 1 |  |  | 114.68 | 50.80 | 155.69 | 142.19 | 41.25 | 169.12 | −55.7 | 35.8 | −71.0 | 18.9 |
1 | Alpha gliadin Bu-BQ806209 |  |  |  |  |  |  |  |  | 80.78 | 81.85 | 73.91 | 74.03 | 66.24 | 3.0 | 4.4 | 0.2 | −10.4 | 80.78 |
1 | Alpha gliadin Bu-BQ807130 |  |  |  |  |  |  |  |  | 96.61 | 107.05 | 114.95 | 125.15 | 119.48 | 135.56 | 10.8 | 19.0 | −4.5 | 8.3 |
19 | Total alpha gliadin |  |  |  |  |  |  |  |  | 2504.83 | 2529.92 | 2906.30 | 3146.79 | 3103.98 | 3435.79 | 1.0 | 16.0 | −1.4 | 9.2 |
2 | Gamma gliadin Bu-1 |  | 1 |  | 2 |  |  |  |  | 205.88 | 217.38 | 179.18 | 223.09 | 212.75 | 176.47 | 5.6 | −13.0 | −4.6 | −20.9 |
1 | Gamma gliadin Bu-2 |  |  |  |  |  |  |  |  | 298.32 | 306.62 | 266.99 | 337.45 | 332.72 | 295.21 | 2.8 | −10.5 | −1.4 | −12.5 |
4 | Gamma gliadin Bu-5 |  | 1 |  |  |  |  |  |  | 795.04 | 816.60 | 738.76 | 798.55 | 802.41 | 719.11 | 2.7 | −7.1 | 0.5 | −9.9 |
3 | Gamma gliadin Bu-6 |  |  |  | 2 |  |  |  |  | 294.02 | 308.72 | 291.19 | 341.59 | 321.44 | 282.37 | 5.0 | −1.0 | −5.9 | −17.3 |
1 | Gamma gliadin Bu-7 |  |  |  |  |  |  |  |  | 109.93 | 115.62 | 119.32 | 117.19 | 110.12 | 126.22 | 5.2 | 8.5 | −6.0 | 7.7 |
2 | Gamma gliadin Bu-11 |  |  |  |  |  |  |  |  | 281.31 | 285.23 | 300.08 | 303.39 | 296.76 | 315.99 | 1.4 | 6.7 | −2.2 | 4.2 |
13 | Total gamma gliadin |  |  |  |  |  |  |  |  | 1984.50 | 2050.18 | 1895.51 | 2121.27 | 2076.21 | 1915.37 | 3.3 | −4.5 | −2.1 | −9.7 |
6 | Omega-5 gliadin | 6 | 6 | 6 | 6 |  |  |  |  | 675.82 | 229.83 | 146.27 | 1153.93 | 237.46 | 140.56 | −66.0 | −78.4 | −79.4 | −87.8 |
2 | Omega-1,2 gliadin D310 |  | 1 |  | 2 |  |  |  |  | 479.80 | 458.24 | 393.76 | 631.49 | 581.38 | 410.86 | −4.5 | −17.9 | −7.9 | −34.9 |
2 | Omega-1,2 gliadin Bu-D510 | 1 | 2 | 1 | 2 |  |  |  |  | 242.54 | 214.06 | 111.87 | 435.25 | 355.39 | 133.28 | −11.7 | −53.9 | −18.3 | −69.4 |
1 | Secalin-like omega gliadin | Â | Â | Â | Â | Â | Â | Â | Â | 37.73 | 41.88 | 49.08 | 49.63 | 52.13 | 56.00 | 11.0 | 30.1 | 5.1 | 12.8 |
11 | Total omega gliadin |  |  |  |  |  |  |  |  | 1435.89 | 944.01 | 700.98 | 2270.30 | 1226.37 | 740.69 | −34.3 | −51.2 | −46.0 | −67.4 |
4 | Total mixed gliadin | Â | Â | Â | Â | Â | Â | Â | Â | 404.96 | 425.58 | 424.86 | 432.88 | 460.34 | 472.80 | 51 | 4.9 | 6.3 | 9.2 |
7 | Total triticin | Â | Â | Â | Â | Â | 1 | Â | 3 | 673.65 | 727.12 | 794.69 | 777.11 | 839.56 | 964.18 | 7.9 | 18.0 | 8.0 | 24.1 |
1 | Farinin Bu-1 |  |  |  |  |  |  |  |  | 58.89 | 55.48 | 68.66 | 41.31 | 33.45 | 39.08 | −5.8 | 16.6 | −19.0 | −5.4 |
3 | Farinin Bu-2 |  |  |  |  |  |  |  |  | 334.76 | 320.74 | 307.56 | 231.53 | 221.26 | 221.18 | −4.2 | −8.1 | −4.4 | −4.5 |
2 | Farinin Bu-3 |  |  |  |  |  |  |  |  | 143.03 | 154.74 | 146.48 | 125.87 | 124.27 | 128.67 | 8.2 | 2.4 | −1.3 | 2.2 |
6 | Total farinin |  |  |  |  |  |  |  |  | 536.68 | 530.95 | 522.70 | 398.71 | 378.98 | 388.94 | −1.1 | 2.6 | −4.9 | 2.5 |
2 | Purinin Bu-1 |  |  |  |  |  | 1 |  |  | 204.59 | 217.28 | 239.48 | 180.15 | 162.16 | 175.04 | 6.2 | 17.1 | −10.0 | −2.8 |
2 | Purinin Bu-2 |  |  |  | 1 |  |  |  |  | 213.30 | 224.44 | 247.53 | 185.90 | 174.15 | 168.17 | 5.2 | 16.0 | −6.3 | −9.5 |
2 | Purinin Bu-3 |  |  |  |  |  |  |  |  | 457.02 | 475.77 | 529.57 | 423.85 | 423.25 | 400.29 | 4.1 | 15.9 | −0.1 | −5.6 |
6 | Total purinin |  |  |  |  |  |  |  |  | 874.92 | 917.50 | 1016.58 | 789.89 | 759.56 | 743.49 | 4.9 | 16.2 | −3.8 | −5.9 |
1 | Globulin-1 |  |  |  |  |  |  |  |  | 41.35 | 41.57 | 52.98 | 58.73 | 58.46 | 70.51 | 0.5 | 28.1 | −0.5 | 20.1 |
5 | Globulin-2 | 1 | 2 | 2 | 2 |  |  |  |  | 115.61 | 105.93 | 102.16 | 104.84 | 98.49 | 91.10 | −8.4 | −11.6 | −6.1 | −13.1 |
4 | Globulin Glo-3 |  | 1 |  | 1 |  |  |  |  | 246.03 | 257.17 | 215.45 | 210.61 | 225.19 | 187.46 | 4.5 | −12.4 | 6.9 | −11.0 |
10 | Total globulin |  |  |  |  |  |  |  |  | 402.98 | 404.67 | 370.59 | 374.18 | 382.14 | 349.08 | 0.4 | −8.0 | 2.1 | −6.7 |
4 | Serpin Bu-1 |  |  |  |  |  | 3 |  |  | 168.10 | 162.31 | 211.52 | 251.48 | 253.04 | 278.30 | −3.4 | 25.8 | 0.6 | 10.7 |
2 | Serpin Bu-2 |  |  |  |  |  | 2 |  |  | 53.48 | 52.28 | 70.47 | 108.94 | 114.09 | 119.34 | −2.2 | 31.8 | 4.7 | 9.6 |
2 | Serpin Bu-3 |  |  |  |  |  |  |  | 1 | 41.23 | 40.67 | 50.63 | 63.64 | 66.61 | 81.15 | −1.4 | 22.8 | 4.7 | 27.5 |
1 | Serpin Bu-4 | Â | Â | Â | Â | Â | 1 | Â | Â | 67.65 | 69.35 | 83.86 | 105.73 | 111.12 | 128.48 | 2.5 | 24.0 | 5.1 | 21.5 |
2 | Serpin Bu-5 |  |  |  |  |  |  |  |  | 83.63 | 83.32 | 103.75 | 126.05 | 125.89 | 152.76 | −0.4 | 24.1 | −0.1 | 21.2 |
2 | Serpin Bu-7 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 59.27 | 62.64 | 63.17 | 74.88 | 70.10 | 73.42 | 5.7 | 6.6 | −6.4 | −2.0 |
13 | Total serpin |  |  |  |  |  |  |  |  | 473.36 | 470.56 | 583.41 | 730.71 | 740.86 | 833.45 | −0.6 | 23.2 | 1.4 | 14.1 |
2 | Beta-amylase Bu-2 |  |  |  |  |  |  |  |  | 102.36 | 93.67 | 101.60 | 99.71 | 98.17 | 99.37 | −8.5 | −0.7 | −1.5 | −0.3 |
3 | Beta-amylase Bu-3 | 1 | 2 | 3 | 1 |  |  |  |  | 117.39 | 99.37 | 100.84 | 136.55 | 87.79 | 98.43 | −15.4 | −14.1 | −35.7 | −27.9 |
5 | Total beta-amylase |  |  |  |  |  |  |  |  | 219.75 | 193.03 | 202.43 | 236.27 | 185.97 | 197.80 | −12.2 | −7.9 | −21.3 | −16.3 |
2 | WMAI Bu-1 |  |  |  |  |  |  |  |  | 624.43 | 660.27 | 661.82 | 469.28 | 488.91 | 456.32 | 5.7 | 6.0 | 4.2 | −2.8 |
1 | WDAI Bu-1 |  |  |  |  |  |  |  |  | 616.66 | 621.14 | 659.08 | 506.27 | 464.64 | 489.27 | 0.7 | 6.9 | −8.2 | −3.4 |
1 | WDAI Bu-3 |  |  |  |  |  |  |  |  | 256.36 | 259.57 | 294.40 | 245.10 | 230.20 | 273.67 | 1.3 | 14.8 | −6.1 | 11.7 |
1 | WDAI Bu-4 |  |  |  |  |  |  |  |  | 179.79 | 194.48 | 206.86 | 130.61 | 113.52 | 126.49 | 8.2 | 15.1 | −13.1 | −3.1 |
1 | WTAI CM1 |  |  |  |  |  |  |  |  | 223.52 | 233.25 | 247.31 | 183.44 | 172.34 | 170.34 | 4.3 | 10.6 | −6.1 | −7.1 |
2 | WTAI CM2 |  |  | 1 |  |  |  |  |  | 1028.76 | 1046.46 | 1023.65 | 760.84 | 682.30 | 707.44 | 1.7 | −0.5 | −10.3 | −7.0 |
2 | WTAI CM3 |  |  |  |  |  |  |  |  | 653.13 | 660.93 | 658.76 | 483.08 | 436.22 | 402.44 | 1.2 | 0.9 | −9.7 | −16.7 |
2 | WTAI CM16 |  |  | 1 | 1 |  |  |  |  | 549.43 | 517.83 | 535.74 | 397.30 | 332.88 | 337.40 | −5.8 | −2.5 | −16.2 | −15.1 |
2 | WTAI CM17 |  |  |  |  |  |  |  |  | 442.72 | 437.00 | 443.39 | 307.31 | 263.48 | 266.70 | −1.3 | 0.1 | −14.3 | −13.2 |
2 | WCI |  |  |  |  |  |  |  |  | 402.00 | 420.67 | 403.68 | 281.27 | 260.35 | 243.91 | 4.6 | 0.4 | −7.4 | −13.3 |
2 | CMX1/CMX3 |  |  |  |  |  |  |  |  | 256.24 | 268.07 | 268.12 | 197.36 | 191.19 | 184.98 | 4.6 | 4.6 | −3.1 | −6.3 |
1 | WASI |  |  |  |  |  |  |  |  | 250.63 | 248.99 | 262.19 | 177.70 | 184.67 | 188.80 | −0.7 | 4.6 | 3.9 | 6.2 |
19 | Total amylase/protease inhibitors |  |  |  |  |  |  |  |  | 5483.67 | 5568.66 | 5664.99 | 4139.56 | 3820.69 | 3847.76 | 1.5 | 3.3 | −7.7 | −7.0 |
32 | Other identified proteins |  | 1 |  | 1 | 1 |  |  |  | 2056.00 | 2108.86 | 2064.67 | 1888.87 | 1871.32 | 1966.62 | 2.6 | 0.4 | −0.9 | 4.1 |