Figure 6
From: Wheat homologs of yeast ATG6 function in autophagy and are implicated in powdery mildew immunity
Knocking down wheat autophagy-related ATG6 genes weakly compromises the broad-spectrum resistance gene Pm21 -triggered resistance response to Blumeria graminis f. sp. tritici ( Bgt ). Barley stripe mosaic virus (BSMV)-treated 92R137/Yangmai 1587 (Pm21) plants of the six-leaf stage were heavily inoculated with Bgt conidia. Fungal structures were stained by trypan blue and visualized by microscopy. (A) Bgt penetration frequency. The number of successfully penetrating conidium with secondary hyphae on the fourth leaves was counted at 7 days after inoculation (dai) with Bgt conidia. Values represent the mean percentage of successfully penetrating Bgt conidia ± SEM calculated from at least 100 interaction sites. CK is from BSMV:GFP-treated 92R137/Yangmai 1587 (Pm21) control plants. Arabic numbers indicate individual BSMV:ATG6-treated 92R137/Yangmai 1587 (Pm21) plants. (B) Representative images of Bgt infection on the fourth leaves of BSMV-treated 92R137/Yangmai 1587 (Pm21) plants at 7 dai. Successfully penetrating conidia with secondary hyphae are indicated by arrows.