Fig. 8
Scatter Loading Plot of principal component (PC) one and two of the principal component analysis (PCA) results obtained from 1H NMR spectral data of the pod based media on which fungi were cultured and scaled to Pareto distribution. Chemical shifts identified for each metabolite are shown in bracket in the figure; the chemical shifts from literature (all from [10] except those for Vanillyl alcohol which is from [26]) are shown in brackets in this figure legend, as follows, next to the numbers (one to nine) that identify the metabolites in the above figure: 1 – Sucrose (Chemical shifts: δ 5.4, δ 4.17), 2 – Glucose (Chemical shifts: δ 5.18, δ 4.57), 3 – Vanillin (Chemical shifts: δ 9.73, δ 7.52, δ 7.49, δ 7.04, δ 3.93), 4 – Glucovanillin (Chemical shifts: δ 9.82, δ 7.62, δ 7.57, δ 7.34, δ 5.19, δ 3.95), 5 - p-hydroxybenzaldehyde (Chemical shifts: δ 9.75, δ 7.85, δ 7.04), 6 - p-hydroxybenzaldehyde glucoside (Chemical shifts: δ 9.84, δ 7.94, δ 7.29, δ 5.01), 7 - Vanillyl alcohol (Chemical shifts: δ 3.75, δ 4.38, δ 5.02, δ 6.71, δ 6.72. δ 6.89, δ 8.79), 8 - bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-isopropyltartrate (Glucoside A) (Chemical shifts: δ 2.20, δ 0.92, δ 0.86) and 9 - bis[4-(β-D-glucopyranosyloxy)-benzyl]-2-(2-butyl)tartrate (Glucoside B) (Chemical shifts: δ 1.90, δ 1.35, δ 1.10, δ 0.84, δ 0.77). Quadrants were labelled with metabolite(s) that predominate in amount in each