Fig. 3

CiNAC4 were induced by abiotic stress and ABA. One-month-old C. intermedia seedlings treated with exogenous ABA (sprayed with 200 μM ABA), cold stress (put into 4 °C incubator), heat stress (put into 42 °C incubator), NaCl (watered with 200 mM NaCl), wounding (2/3 of the total leaves were pierced with tweezers), spray (sprayed with water, used as the control of ABA treatment), dehydration stress (cleaned the soil on the root and put on the filter paper), or drought stress (withholding water) were harvested at the indicated time points. Expression values were calculated using 2^-ΔΔCT method and CiEF1a as endogenous control. Two independent biological replicates were performed with similar result. Three technical replicates of each biological replicate were analyzed in quantitative real-time PCR analysis