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Fig. 3 | BMC Plant Biology

Fig. 3

From: Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida)

Fig. 3

Silencing of RhAG in rose flowers. Three month old rose plants were infiltrated with A.tumefaciens containing a TRV control (TRV, pTRV1 + pTRV2), or TRV carrying an RhAG fragment (TRV-RhAG, pTRV1 + pTRV2- RhAG). a Phenotype of the TRV control (left) and RhAG-silenced (right) flowers at stage 10. b Quantitative RT-PCR analysis of RhAG expression in TRV control and RhAG-silenced petals. The expression level of RhAG in the TRV19 control was set to 1.0. RhTCTP was used as the internal control. Floral buds at stage 3 were used. Values are means ± SD (n = 3). c Petals from TRV flowers (left) and RhAG-silenced flowers (right). d Petal (left) and stamen (right) numbers of TRV flowers and RhAG-silenced flowers (n = 5). Asterisks indicate significant differences calculated using the t test (**p < 0.01; *p < 0.05). Scale bars = 1 cm

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BMC Plant Biology

ISSN: 1471-2229

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