Fig. 8
From: Genetics and physiology of cell wall polysaccharides in the model C4 grass, Setaria viridis spp
Micrographs of main root transverse sections. a Bright-field light micrograph of a toluidine blue-stained survey section. b-d Fluorescence light micrographs using b an antibody conjugated to Alexafluor 488, c Calcofluor White MR2, d a secondary antibody only as negative control. (1,3;1,4)-β-Glucan is indicated by green fluorescence. e-h TEM micrographs of sections immunogold labelled with the (1,3;1,4)-β-glucan antibody BG-1. Walls of cells in the e epidermis, f cortex, g endodermis and h vascular bundle. E, epidermis; C, cortex; En, endodermis; VB, vascular bundle; Mxy, metaxylem. Magnification: a-d 100 x. Scale bars: a-d 100 μm; e-h 0.5 μm. Exposure time for b = 650 ms