Fig. 9

The galt single mutants demonstrate reduced inhibition of pollen tube growth in the presence of β-Gal-Yariv reagent. a Representative images of pollen tubes from WT, galt1, galt3, galt4, and galt6 mutants after 16 h in pollen germination medium, and b in pollen germination medium supplemented with 30 μM α-Gal-Yariv, and c in pollen germination medium supplemented with 30 μM β-Gal-Yariv reagent. Bar = 50 μm. d Pollen tube lengths from WT, galt1-galt6 mutants, and galt2galt5 double mutants were measured over 16 h in the pollen germination medium supplemented with 30 μM β-Gal-Yariv reagent. Twenty flowers from each genotype and 25 pollen tubes from each flower were measured using Image J software. The experiment was done in triplicate, and the asterisks indicate mean values significantly different from the WT (Dunnett’s test, *P <0.05; **P <0.01; ***P <0.001)