Table 1 Primers used in this study
From: Overexpression of Vitreoscilla hemoglobin increases waterlogging tolerance in Arabidopsis and maize
Primers | Sequence (5´-3´) | Usage |
|---|---|---|
VHb c-forward | ATGTTAGACCAGCAAACCATTAA | RT-PCR for full-length CDS of VHb |
VHb c-reverse | TTATTCAACCGCTTGAGCGTACA | RT-PCR for full-length CDS of VHb |
VHb forward | CCAGCAAACCATCAACATCA | Amplifying the VHbgene |
VHb reverse | CGTAAAGGTCAGCCTCAACC | Amplifying the VHb gene |
VHb q-forward | GACTATCAACATCATCAAGGC | qRT-PCR for VHb gene |
VHb q-reverse | GACATCAGCAATAACACCGTA | qRT-PCR for VHb gene |
ADH1 forward | GTGGCTGTTTTCGGTTTAGGA | qRT-PCR for maize ADH1 gene |
ADH1 reverse | ACTGGCTTGTTGTGGTCTTTT | qRT-PCR for maize ADH1 gene |
Ara-Actin1 forward | CCCCTGCTATGTATGTGGCTAT | qRT-PCR for ArabidopsisActin1 gene |
Ara-Actin1 reverse | GACAATTTCACGCTCTGCTGT | qRT-PCR for ArabidopsisActin1 gene |
Maize-Actin1 forward | TGTTGCTATCCAGGCTGTTCT | qRT-PCR for maize Actin1 gene |
Maize-Actin1 reverse | TCATTAGGTGGTCGGTGAGGT | qRT-PCR for maize Actin1 gene |