Fig. 5
From: Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8
UVR8 does not bind to nucleosomes in vitro. a Purified recombinant RCC1 and UVR8 (− and + UV-B treatment) were electrophoretically separated on SDS-PAGE together or in the absence of reconstituted nucleosomes (Nucl.) and stained by Coomassie Blue. Samples were boiled or non-boiled before loading the gel. b Electrop horetic mobility gel shift assay of nucleosome binding by RCC1 and UVR8. Between 0.15 - 10 μM RCC1 and 0.15 - 40 μM UVR8 (− and + UV-B treatment) were added to reconstituted mononucleosomes. Binding was assayed by EMSA and visualized by ethidium bromide staining