Fig. 2
AtNRP1, AtNRP2, ANAC036 and gVPE are induced by osmotic and ER stresses. Total RNA was isolated from 15 days–old Arabidopsis seedlings that had been treated with PEG (10 % w/v) or Tunicamycin (2,5 μg/mL) for 2 h, 6 h, 12 h and 24 h. H2O was used as control for PEG and DMSO for Tunicamycin. The transcript levels of selected genes, as indicated, were quantified by qRT–PCR. Gene expression was calculated using the 2-ΔΔCt method and UBQ5 as endogenous control. cDNAs were obtained from five biological replicates and validated individually. RAB18 and CNX are osmotic stress and ER stress gene markers, respectively. (S.E., n = 5 biological replicates). Col denotes Col–0 (wild–type) line and BiPDox is Arabdidopsis transgenic lines ectopically expressing the soyBiPD gene. a PEG induction of RAB18. b Tunicamycin induction of calnexin (CNX). c PEG induction of AtNRP1, AtNRP2 and ANAC036. d Tunicamycin induction of AtNRP1, AtNRP2 and ANAC036. e PEG induction of VPE. f Tunicamycin induction of VPE