Fig. 1

RP-HPLC analysis of anthocyanins (a) and polyphenolic profile (b) from alcoholic extracts obtained by V. vinifera (cv. Limberger) suspension cell cultures. GSC (dotted line) and RSC (solid line) were grown under light conditions for 6 days. Their metabolite content was determined at day 6. Chromatographic profiles of anthocyanidin glucosides, cyanidin and malvidin, as well as their respective substituted derivatives, are presented. Malvidin glucoside (dashed line) was used as a standard (Panel a). The identification was obtained by mass spectrometry analysis on chromatographic peaks detected at 520 nm. Similar analysis was performed at 320 nm (Panel b) and the retrieved flavonoids were identified as follows: 1) gallic acid; 2) quercetin-diglucoside; 3) quercetin-glucoside; 4) quercetin-glucuronide methyl ester; 5) kaempferol-glucoside; 6) quercetin; 7) kaempferol. Data are representative of three different experiments