PCR marker | Name | Primer (5’ → 3’) | Target allele(s) | Expected pro-duct size (bp) | Annealing temp. (°C) | Reference. |
---|---|---|---|---|---|---|
VRN-A1 markera | Vrn1AF | GAAAGGAAAAATTCTGCTCG | VRN-A1f | 655 | 55 | [13] |
Int1R | GCAGGAAATCGAAATCGAAG | VRN-A1f-del | ||||
VRN-A1f-ins | ||||||
VRN-A1f-del/ins | ||||||
VRN-A1 Non-deletion | Intr1/C/F | GCACTCCTAACCCACTAACC | VRN-A1f | 1068 | 56 | [14] |
Intr1/AB/R | TCATCCATCATCAAGGCAAA | VRN-A1f-ins | ||||
VRN-A1 insertion | Intr 1 | ATCATCTTCTCCACCAAGGG | VRN-A1f | 1480 | 50 | [15] |
Intr1insR | AATGAACAGCACGGAAACAG | VRN-A1f-ins | 1910 | Â | Â | |
VRN-A1f-del/ins | Â | Â | Â | |||
mitef | CAGGTAAGGTATGAGGTGAC | VRN-A1f-ins | 370 | 52 | - | |
miter | GATTCCAAATGAGAAGATGAGG | VRN-A1f-del/ins | Â | Â | Â | |
VRN-A1 deletion of 2.7Â kb | Intr1/A/F2 | AGCCTCCACGGTTTGAAAGTAA | VRN-A1f-del | ~2500 | 56 | [14] |
Intr4 | GCGCCATTAGGGAGGCACTT | VRN-A1f-del/ins | Â | Â | Â | |
delf | ACATGTAAGCAGATCCTATCGA | VRN-A1f-del | 450 | 53 | - | |
delr | TGCTTTAGATCTTTCTTCACGG | VRN-A1f-del/ins | Â | Â | Â | |
VRN-G1 markera | ||||||
for T. araraticum/ | P1 | TACCCCTGCTACCAGTGCCT | VRN-G1 | 900 | 55 | [23] |
T. timopheevii | P5 | GGCCAACCCTACACCCCAAG | VRN-G1a | 1100 | Â | Â |
for Ae. speltoides | P8 | CTAGGACTGGCGAGTATCTT | VRN-1 | 900 | 55 | - |
P10 | GAGAACCGGGCCAACCCTAC | Â | Â | Â | Â | |
VRN-G1 Non-deletion | ||||||
for T. araraticum/ | Ex1/C/F | GTTCTCCACCGAGTCATGGT | VRN-G1 | 1530 | 56 | [14] |
T. timopheevii | Intr1/B/R4 | CAAATGAAAAGGAATGAGAGCA | VRN-G1a | Â | Â | Â |
for Ae. speltoides | Ex1/C/F | GTTCTCCACCGAGTCATGGT | VRN-1 | 1530 | 60 | [14] |
Intr1/D/R4 | AAATGAAAAGGAACGAGAGCG | Â | Â | Â | Â |