Fig. 2

Isolation of the rice osacos12 mutant with complete male sterility. a The gene structure and position of the nucleotide change in osacos12. The black boxes indicate exons. b-d The wild-type (WT) plant, osacos12 mutant and complementation plant after the heading stage. e Comparison of the WT plant (left) and osacos12 mutant panicles (right) at the heading stage. f The spikelets of the WT plant (left) and an osacos12 mutant (right) after removing the palea. g The anther of a WT plant (left) and an osacos12 mutant (right). h Alexander staining of the WT plant (left) and an osacos12 mutant anther (right). i and j Identification of the OsACOS12 gene in a WT plant and osacos12 mutant by sequencing (position 1000). Bars = 100 μm in e-g and 200 μm in h