Fig. 3

Relative expression level of clade V MLO genes in different cucumber tissues in response to Podosphaera xanthii inoculation. Relative transcript abundances in three tissues (a) hypocotyl, b cotyledon and c leaf) of PM-susceptible cucumber genotype ‘Sheila’ before and at 4, 6, 8 and 24 h post inoculation with P. xanthii were determined using qRT-PCR. Data were normalized relative to the geometric average of the Ct values of reference genes Ef-α and TIP41, and subsequently normalized relative to the average dCt value at 0 hpi for each gene/tissue combination. Relative transcript abundances were calculated as 2^-ddCt. Each bar shows the relative expression of four to eight biological replicates on a logarithmic scale. Error bars indicate standard error of the mean. Different letters above bars indicate significant differences between time points (ANOVA with Bonferroni post hoc tests, P < 0.05)