Fig. 1

a The construction of the isolation plots for R. glutinosa plants. The soil was first removed from the walkways between any two plots (left and right sides), to a depth greater than 1 m, as was soil from the other two sides of each plot. The soil that was dug from these locations was moved away from the plots. The separation walls were then built on the four sides of each plot using brick (Step 2), and the separation walls were then evenly covered with impermeable membranes (Step 3) that were placed tightly against the walls (Step 4). After the separation walls of each plot were built, the soil was backfilled into the plots and walkways (Step 5). Finally, the surfaces of the plots were levelled and made consistent with each other (Step 6). b Construction of R. glutinosa transcriptome libraries and c assembly of unigene sequences generated from different libraries