Fig. 1

Microarray analysis of dormant and after-ripened seeds after 24 h of imbibition of five genotypes with differing dormancy levels; Ler, NILDOG1, NILDOG2, NILDOG3 and NILDOG6. a After-ripening requirement of the five genotypes. On the right graphical representations of the NILs are depicted showing the 5 chromosomes with the introgressed regions (in red) in an otherwise Ler background (in green). b Venn diagrams showing the number of genes that are differentially expressed (P < 0.0001) in dormant (D-up) and after ripened (AR-up) 24-h imbibed seeds of different genotypes. For each genotype the total number of differential expressed genes is indicated between brackets. In the intersection of all genotypes the number of genes that are investigated in this study are presented, 46 and 25 for the D-up and AR-up set, respectively. c Heat map consisting of 245 NILDOG1 D-up genes (P < 0.0001). The significance of these genes in the other genotypes is indicated, the white color indicates the genes that are not significantly different in the other genotypes (P < 0.01). d Heat map consisting of 159 NILDOG1 AR-up genes (P < 0.0001). The significance of these genes in the other genotypes is indicated, the white color represents the genes that are not significantly different in the other genotypes (P < 0.01). e Germination behaviour of freshly harvested seeds of Ler, dog1 and NILDOG1. f Box plot showing the expression of the 45 D-up genes in freshly harvested imbibed Ler, dog1 and NILDOG1 seeds (expression data taken from Dekkers et al. [16]). g Box plot showing the expression of the 25 D-up genes in freshly harvested imbibed Ler, dog1 and NILDOG1 seeds