Table 1 Proteins with increased abundance in “Dongcha11” shoots newly germinated in winter compared to spring tender shoots
Spot No. | Protein name | Species | Accession | Ratio | Mascot score | MS coverage% | Theoretical Mr.(kDa)/pI | Expressed Mr.(kDa)/pI |
|---|---|---|---|---|---|---|---|---|
Photosynthesis | ||||||||
1 | Ribulose 1,5-bisphosphate carboxylase | Aralidium pinnatifidu | AAG24624 | >100 | 80 | 5 | 52.13/6.2 | 14.46/4.07 |
2 | Ribulose 1,5-bisphosphate carboxylase | Aralidium pinnatifidu | AAG24624 | 75.93 | 76 | 5 | 52.13/6.2 | 14.74/4.46 |
3 | Ribulose bisphosphate carboxylase large chain | Acacia farnesiana | P93998 | 19.9 | 415 | 12 | 50.86/6.2 | 20.22/6.00 |
4 | Ribulose bisphosphate carboxylase large chain | Acacia farnesiana | P93998 | 13.38 | 427 | 7 | 50.86/6.2 | 18.93/5.03 |
5 | Ribulose bisphosphate carboxylase large chain | Akania bidwillii | Q07281 | 5.62 | 126 | 8 | 52.31/6.2 | 29.14/6.82 |
6 | Ribulose bisphosphate carboxylase large chain | Akania bidwillii | Q07281 | 3.81 | 355 | 13 | 52.31/6.2 | 29.21/6.73 |
7 | Ribulose bisphosphate carboxylase large chain | Begonia metallica X sanguinea | P28383 | 3.02 | 308 | 15 | 49.17/6.6 | 28.32/6.43 |
8 | RuBisCO large subunit-binding protein subunit alpha, chlorop | Brassica napus (Rape) | P21239 | 2.09 | 344 | 8 | 57.71/4.7 | 59.74/4.07 |
9 | Plastocyanin | Capsella bursa-pastoris | P00294 | 2.51 | 65 | 24 | 10.43/4.1 | 14.11/3.54 |
10 | ATP synthase delta chain, chloroplastic | Spinacia oleracea | P11402 | 5.77 | 47 | 6 | 27.66/5.7 | 17.54/5.76 |
Cell structure | ||||||||
11 | Putative histone H3-like 5 | Vitis vinifer | Q9FKQ3 | >100 | 52 | 14 | 15.64/11.9 | 15.19/6.22 |
12 | Profilin-1 | Ricinus communis | O82572 | 2.09 | 84 | 29 | 14.31/4.4 | 12.31/3.61 |
13 | Profilin-A | Camellia sinensis | Q9FUD1 | 2.75 | 71 | 9 | 14.35/4.8 | 12.72/3.73 |
14 | Histone H4 | Arabidopsis thaliana | P59259 | >100 | 42 | 13 | 11.40/12.0 | 13.24/6.04 |
15 | Histone H2A.1 | Triticum aestivum | P02275 | >100 | 52 | 8 | 15.58/11.1 | 16.08/6.49 |
Protein synthesis & destination | ||||||||
16 | Predicted protein | Populus trichocarpa | XP_002301464 | 6.5 | 145 | 9 | 29.47/10.0 | 17.32/4.19 |
17 | Predicted protein | Populus trichocarpa | XP_002301464 | 2.09 | 161 | 10 | 29.47/10.0 | 17.21/4.42 |
18 | Proteasome subunit beta type-6 | Arabidopsis thaliana | Q8LD27 | 3.89 | 51 | 6 | 25.19/5.2 | 22.22/5.23 |
19 | Eukaryotic initiation factor 4A-1 | Oryza sativa Japonica | P35683 | >100 | 59 | 9 | 47.34/5.3 | 45.58/5.79 |
Transporters | ||||||||
20 | Hypothetical protein | Camellia sinensis | AEC10968 | 6.97 | 99 | 8 | 24.67/10.4 | 19.54/4.36 |
21 | Phosphatidylglycerol/phosphatidylinositol transfer protein p | Camellia sinensis | AEC10983 | 11.57 | 139 | 25 | 16.96/4.8 | 12.47/4.15 |
Disease/defense | ||||||||
22 | Putative In2.1 protein | Triticum aestivum | CAA76758 | 9.94 | 56 | 4 | 27.25/5.3 | 26.48/4.35 |
23 | Peptide methionine sulfoxide reductase | Solanum lycopersicum | P54153 | 10.56 | 88 | 7 | 22.25/6.1 | 19.72/5.61 |
Protein of unknown functions | ||||||||
24 | unknown | Picea sitchensis | ADE77382 | 3.53 | 91 | 4 | 37.84/5.8 | 32.90/4.11 |