Fig. 1

The maximum likelihood (ML) phylogenetic tree reconstruction using precursor miRNA family sequences from Arabidopsis (ath), grape (vvi), popar (ptc), and hickory (cca). MUSCLE alignment and ML were used for tree generation. The phylogenetic tree for miR167 (a), miR171 (b), miR156 (c), miR166 (d), miR169 (e), miR397 (f). The miRBase accession numbers as follows: ath-MIR167a (MI0000208), ath-MIR167b (MI0000209), ath-MIR167c (MI0001088), ath-MIR167d (MI0000975), ptc-MIR167a (MI0002235), ptc-MIR167b (MI0002236), ptc-MIR167c (MI0002237), ptc-MIR167d (MI0002238), ptc-MIR167e (MI0002239), ptc-MIR167f (MI0002240), ptc-MIR167g (MI0002241), ptc-MIR167h (MI0002242), vvi-MIR167a (MI0006515), vvi-MIR167b (MI0006516), vvi-MIR167c (MI0006517), vvi-MIR167d (MI0006518), vvi-MIR167e (MI0006519), ath-MIR171a (MI0000214), ath-MIR171b (MI0000989), ath-MIR171c (MI0000990), ptc-MIR171a (MI0002277), ptc-MIR171b (MI0002278), ptc-MIR171c (MI0002279), ptc-MIR171d (MI0002280), ptc-MIR171e (MI0002281), ptc-MIR171f (MI0002282), ptc-MIR171g (MI0002283), ptc-MIR171h (MI0002284), ptc-MIR171i (MI0002285), ptc-MIR171j (MI0007034), ptc-MIR171k (MI0005114), ptc-MIR171l (MI0007032), ptc-MIR171m (MI0007033), vvi-MIR171a (MI0006536), vvi-MIR171b (MI0006537), vvi-MIR171c (MI0006538), vvi-MIR171d (MI0006539), vvi-MIR171e (MI0006540), vvi-MIR171f (MI0006541), vvi-MIR171g (MI0007950), vvi-MIR171h (MI0006542), vvi-MIR171i (MI0006543), vvi-MIR171j (MI0031743), ptc-MIR156a (MI0002184), ptc-MIR156b (MI0002185), ptc-MIR156c (MI0002186), ptc-MIR156d (MI0002187), ptc-MIR156e (MI0002188), ptc-MIR156f (MI0002189), ptc-MIR156g (MI0002190), ptc-MIR156h (MI0002191), ptc-MIR156i (MI0002192), ptc-MIR156j (MI0002193), ptc-MIR156k (MI0002194), ptc-MIR156l (MI0022040), ath-MIR156a (MI0000178), ath-MIR156b (MI0000179), ath-MIR156c (MI0000180), ath-MIR156d (MI0000181), ath-MIR156e (MI0000182), ath-MIR156f (MI0000183), ath-MIR156g (MI0001082), ath-MIR156h (MI0001083), ath-MIR156i (MI0019232), ath-MIR156j (MI0019234), vvi-MIR156a (MI0006485), vvi-MIR156b (MI0006486), vvi-MIR156c (MI0006487), vvi-MIR156d (MI0006488), vvi-MIR156e (MI0006489), vvi-MIR156f (MI0006490), vvi-MIR156g (MI0006491), vvi-MIR156h (MI0007939), vvi-MIR156i (MI0006492), ptc-MIR166o (MI0002232), ptc-MIR166p (MI0002233), ptc-MIR166q (MI0002234), vvi-MIR166a (MI0006507), vvi-MIR166b (MI0006508), vvi-MIR166c (MI0006509), vvi-MIR166d (MI0006510), vvi-MIR166e (MI0006511), vvi-MIR166f (MI0006512), vvi-MIR166g (MI0006513), vvi-MIR166h (MI0006514), ath-MIR169a (MI0000212), ath-MIR169b (MI0000976), ath-MIR169c (MI0000977), ath-MIR169d (MI0000978), ath-MIR169e (MI0000979), ath-MIR169f (MI0000980), ath-MIR169g (MI0000981), ath-MIR169h (MI0000982), ath-MIR169i (MI0000983), ath-MIR169j (MI0000984), ath-MIR169k (MI0000985), ath-MIR169l (MI0000986), ath-MIR169m (MI0000987), ath-MIR169n (MI0000988), ptc-MIR169a (MI0002245), ptc-MIR169b (MI0002252), ptc-MIR169c (MI0002253), ptc-MIR169d (MI0002254), ptc-MIR169e (MI0002255), ptc-MIR169f (MI0002256), ptc-MIR169g (MI0002257), ptc-MIR169h (MI0002258), ptc-MIR169i (MI0002259), ptc-MIR169j (MI0002260), ptc-MIR169k (MI0002261), ptc-MIR169l (MI0002262), ptc-MIR169m (MI0002263), ptc-MIR169n (MI0002264), ptc-MIR169o (MI0002265), ptc-MIR169p (MI0002266), ptc-MIR169q (MI0002267), ptc-MIR169r (MI0002268), ptc-MIR169s (MI0002269), ptc-MIR169t (MI0002270), ptc-MIR169u (MI0002271), ptc-MIR169v (MI0002272), ptc-MIR169w (MI0002273), ptc-MIR169x (MI0002274), ptc-MIR169y (MI0002275), ptc-MIR169z (MI0002276), ptc-MIR169aa (MI0002246), ptc-MIR169ab (MI0002247), ptc-MIR169ac (MI0002248), ptc-MIR169ad (MI0002249), ptc-MIR169ae (MI0002250), ptc-MIR169af (MI0002251), ptc-MIR169ag (MI0022041), vvi-MIR169a (MI0006521), vvi-MIR169b (MI0007940), vvi-MIR169c (MI0006523), vvi-MIR169d (MI0006524), vvi-MIR169e (MI0006525), vvi-MIR169f (MI0006526), vvi-MIR169g (MI0006527), vvi-MIR169h (MI0007941), vvi-MIR169i (MI0007942), vvi-MIR169j (MI0006528), vvi-MIR169k (MI0006529), vvi-MIR169l (MI0007943), vvi-MIR169m (MI0006530), vvi-MIR169n (MI0007944), vvi-MIR169o (MI0007945), vvi-MIR169p (MI0006531), vvi-MIR169q (MI0007946), vvi-MIR169r (MI0006532), vvi-MIR169s (MI0006533), vvi-MIR169t (MI0006534), vvi-MIR169u (MI0006535), vvi-MIR169v (MI0007947), vvi-MIR169w (MI0007948), vvi-MIR169x (MI0007949), vvi-MIR169y (MI0006522), ath-MIR397a (MI0001015), ath-MIR397b (MI0001016), ptc-MIR397a (MI0002332), ptc-MIR397b (MI0002333), ptc-MIR397c (MI0002334), vvi-MIR397a (MI0007956)