Fig. 2From: Functional characterization of soybean strigolactone biosynthesis and signaling genes in Arabidopsis MAX mutants and GmMAX3 in soybean nodulationFunction analysis of GmMAX1a expressed in Arabidopsis. Complementation of GmMAX1a in atmax1–1 mutant and overexpression in the wild-type Col-0 were done to compare the functions of GmMAX1a with AtMAX1. a The seedlings of atmax1–1, Col-0, and atmax1–1 complementation plant with GmMAX1a (atmax1/GmMAX1a) with different primary root lengths (left panel). Quantification of shoot height was conducted and calculated on at least three independent lines of more than 20 plants (right panel). b Mature plants of Col-0, atmax1–1 mutant, complementation (atmax1/GmMAX1a), and GmMAX1a overexpression lines (left panel). The numbers of branches were determined and calculated on at least three independent lines of more than 20 plants (right panel). c The differences between seedlings (top panel) and leaf shapes (bottom panel) of Col-0, atmax1–1, complementation of (atmax1–1/GmMAX1a) and GmMAX1a-OE plants (left panel). Ratios of leaf length to leaf width were collected on 2-week-old seedlings (right panel). Data represent means ± s.d (n = 20 plants for each genotyping). d Silique lengths of Col-0, atmax1–1, and complementation of (atmax1/GmMAX1a) plants (left panel). Quantification of silique length (cm) of Col-0, atmax1–1, complementation of (atmax1/GmMAX1a) and GmMAX1a-OE plants (right panel). Data represent means ± s.d (n = 20 plants for each genotyping). Data are expressed as means ± s.d. from 3 independent experiments with biological replicates. Asterisks indicate significant difference according to a Student’s t-test (**P < 0.001, * P < 0.05)Back to article page