Fig. 1
From: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping
Structure of two Universal probes (UPs) used in Amplifluor SNP markers. Two UPs labelled with FAM (a) and with HEX/VIC (b) are shown. The five major elements of each UP are indicated by numbers in turn: (1) Fluorophores at the 5′-end; (2, 3) Hairpin with stem and loop, respectively; (4) Modified oligonucleotide Thymine (T) indicated by the asterisk, with Black hole quencher 1 (BHQ1), shown in Bold; (5) A ‘tail’ specific for each UP in the 3′-end, shown in Italics. Figure modified and adapted from [9, 10]